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Knockdown of SUMO1P3 represses tumor growth and invasion and enhances radiosensitivity in hepatocellular carcinoma

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Abstract

Hepatocellular carcinoma (HCC) is one of the most common types of malignant tumors with high recurrence and metastasis rates. Radiotherapy represents a major therapeutic option for HCC patients. However, the efficacy of radiotherapy has been limited due to the development of intrinsic radioresistance of the tumor cells. Small ubiquitin-like modifier 1 pseudogene 3 (SUMO1P3), one member of SUMO pseudogene family, is a novel identified lncRNA that was originally identified to be upregulated in gastric cancer. However, the detailed roles of SUMO1P3 in HCC development remain to be elucidated. Here, the expression of SUMO1P3 in HCC tissues and cells was examined by qRT-PCR. Cell proliferation, colony formation ability, invasion ability, apoptosis, and radiosensitivity were detected by MTT assay, colony formation assay, cell invasion assay, flow cytometry analysis, and survival fraction assay, respectively. We found that SUMO1P3 was significantly upregulated in HCC tissues and cells. Besides, SUMO1P3 was highly expressed in HCC patients with higher TNM stage. Furthermore, SUMO1P3 knockdown markedly suppressed cell proliferation, colony formation ability, and cell invasiveness, promoted apoptosis, and enhanced radiosensitivity of HCC cells. We concluded that the knockdown of SUMO1P3 repressed tumor growth, invasion, promoted apoptosis, and enhanced radiosensitivity in HCC, providing evidence that SUMO1P3 might be a potential novel biomarker and a therapeutic target for HCC.

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Abbreviations

HCC:

Hepatocellular carcinoma

SUMO1P3:

Small ubiquitin-like modifier 1 pseudogene 3

LncRNAs:

Long non-coding RNAs

WHO:

World Health Organization

TNM:

Tumor-node-metastasis

DMEM:

Dulbecco’s Modified Eagle’s Medium

FBS:

Fetal bovine serum

qRT-PCR:

Quantitative real-time polymerase chain reaction

cDNA:

Complementary DNA

DMSO:

Dimethyl sulfoxide

ANOVA:

One-way analysis of variance

GAS5:

LncRNA growth arrest-specific 5

CCAT1:

LncRNA colon cancer-associated transcript-1

CRNDE:

LncRNA colorectal neoplasia differentially expressed

HMGB1:

High-mobility group box 1 protein

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Authors and Affiliations

  1. Department of Infectious Diseases, the First Affiliated Hospital of Zhengzhou University, Νo. 1 Jianshe East Road, Zhengzhou, 450000, Henan, People’s Republic of China

    Yan Zhou, Ping He, Xuhua Xie & Changyu Sun

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  1. Yan Zhou
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  2. Ping He
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  3. Xuhua Xie
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  4. Changyu Sun
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Corresponding author

Correspondence to Changyu Sun.

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The authors declare no competing or financial interests.

Ethical approval

This study was approved by the Ethics Committee of Zhengzhou University and written informed consent was obtained for the use of tissue samples from all participants enrolled in this study.

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Zhou, Y., He, P., Xie, X. et al. Knockdown of SUMO1P3 represses tumor growth and invasion and enhances radiosensitivity in hepatocellular carcinoma. Mol Cell Biochem 450, 125–134 (2019). https://doi.org/10.1007/s11010-018-3379-8

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  • DOI: https://doi.org/10.1007/s11010-018-3379-8

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