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Association of COL4A1 (rs605143, rs565470) and CD14 (rs2569190) genes polymorphism with coronary artery disease

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Abstract

Coronary artery disease (CAD) is the leading cause of death worldwide and it is basically caused by atherosclerosis. The atherosclerotic process includes complex events and each one involves a specific biological pathway and different genes. According to World Health Organization report, Cardiovascular diseases will be the largest cause of death and disability by 2020, with an estimated 2.6 million Indians predicted to die due to CAD predominantly with myocardial infarction. Genetic factors are estimated to contribute 30–60% of the CAD risk. The aim of this study is to investigate the association of COL4A1 and CD14 genes polymorphism with CAD. This study included 345 subjects, 185 CAD cases and 160 healthy controls. Single-nucleotide polymorphisms were evaluated by polymerase chain reaction and restriction fragment length polymorphism. Alleles and genotype frequencies between cases and controls were compared using χ2 and Student’s t tests. Odds ratios and 95% confidence intervals were calculated by logistic regression to assess the relative association between disease and genotypes. In this study, CD14 (rs2569190), CC (P = 0.008) genotypes, and C allele (P = 0.007) were found to be a positive risk factor, while TT genotype (P = 0.045) and T allele (P = 0.007) as negative risk factor for CAD. Significant differences were not observed in COL4A1 (rs605143 and rs565470) gene polymorphism with CAD. It seems that CD14 gene polymorphism might be associated with the risk of CAD, whereas COL4A1 gene polymorphism was not found to confer any risk of CAD.

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References

  1. Bhatia SK (2010) Biomaterials for clinical applications (Online-Ausg. ed.). Springer, New York, p 23. ISBN 9781441969200

    Book  Google Scholar 

  2. Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V et al (2012) Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 380(9859), 2095–2128

    Article  PubMed  Google Scholar 

  3. Finegold JA, Asaria P, Francis DP (2012) Mortality from ischaemic heart disease by country, region, and age: statistics from World Health Organisation and United Nations. Int J Cardiol 168(2):934–945

    Article  PubMed  Google Scholar 

  4. Murray CJ, Lopez AD (1997) Alternative projections of mortality and disability by cause 1990–2020: global burden of disease study. Lancet 349:1498–1504

    Article  PubMed  CAS  Google Scholar 

  5. Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW (2010) Effect of intensive lifestyle changes on endothelial function and inflammatory markers of atherosclerosis. Am J Cardiol 105(3):362–367

    Article  PubMed  CAS  Google Scholar 

  6. Robert Roberts MD (2014) Genetics of coronary artery disease: an update. Methodist Debakey Cardiovasc J 10(1):7–12

    Article  PubMed  PubMed Central  Google Scholar 

  7. Emanuel BS, Sellinger BT, Gudas LJ, Myers JC (1986) Localization of the human procollagenalpha-1(IV) gene to chromosome 13q34 by in situ hybridization. Am J Hum Genet 12:38–44

    Google Scholar 

  8. Kalluri R (2003) Basement membranes: structure, assembly and role in tumor angiogenesis. J Nat Rev 12:422–433

    Article  CAS  Google Scholar 

  9. LeBleu VS, Macdonald B, Kalluri R (2007) Structure and function of basement membranes. Exp Biol Med 12:1121–1129

    Article  CAS  Google Scholar 

  10. Urabe N, Naito I, Saito K (2002) Basement membrane type IV collagen molecules in the choroid plexus, pia mater and capillaries in the mouse brain. Arch Histol Cytol 12:133–143

    Article  Google Scholar 

  11. Yamada Y, Kato K, Oguri M et al (2008) Genetic risk for myocardial infarction determined by polymorphisms of candidate genes in a Japanese population. J Med Genet 45:216–221

    Article  PubMed  CAS  Google Scholar 

  12. Gould DB, Phalan FC, van Mil SE et al (2006) Role of COL4A1 in small-vessel disease and hemorrhagic stroke. N Engl J Med 354:1489–1496

    Article  PubMed  CAS  Google Scholar 

  13. Gould DB, Phalan FC, Breedveld GJ et al (2005) Mutations in Col4a1 cause perinatal cerebral hemorrhage and porencephaly. Science 308:1167–1171

    Article  PubMed  CAS  Google Scholar 

  14. Adi D, Xie X, Y-T M, Z-Y Fu, Y-N Yang, Li X-M, et al (2013) Association of COL4A1 genetic polymorphisms with coronary artery disease in Uygur population in Xinjiang, China. Lipids Health Dis 12:153

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  15. Tapping R, Tobias P (2000) Soluble CD14-mediate cellular response to lipopolysaccharide. Chem Immunol 74:108 – 121

    Article  PubMed  CAS  Google Scholar 

  16. Baldini M, Lohman IC, Halonen M, Erickson RP, Holt PG, Martinez FD (1999) A Polymorphism in the 5′ flanking region of the CD14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E. Am J Respir Cell Mol Biol 20:976–983

    Article  PubMed  CAS  Google Scholar 

  17. Hohda S, Kimura A, Sasaoka T, Hayashi T, Ueda K, Yasunami M et al (2003) Association study of CD14 polymorphism with myocardial infarction in a Japanese population. Jpn Heart J 44(5):613 – 22

    Article  PubMed  CAS  Google Scholar 

  18. Shimada K, Watanabe Y, Mokuno H et al (2000) Common polymorphism in the promoter of the CD14 monocyte receptor gene is associated with acute myocardial infarction in Japanese men. Am J Cardiol 86:682–684

    Article  PubMed  CAS  Google Scholar 

  19. Deloukas P (2013) Large-scale association analysis identifies new risk loci for coronary artery disease. Nat Genet 45:25–33

    Article  PubMed  CAS  Google Scholar 

  20. Tu X (2013) The IL-33-ST2L pathway is associated with coronary artery disease in a Chinese Han population. Am J Hum Genet 93:652–660

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  21. Xu C (2014) Candidate pathway-based genome-wide association studies identify novel associations of genomic variants in the complement system associated with coronary artery disease. Circ Cardiovasc Genet 7:887–894

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  22. Soininen R, Haka-Risku T, Prockop DJ, Tryggvason K (1987) Complete primary structure of the alpha 1-chain of human basement membrane (type IV) collagen. FEBS Lett 225(1–2):188–194

    PubMed  CAS  Google Scholar 

  23. BioGPS—your Gene Portal System. http://www.biogps.org

  24. Guicheng P, Maohuan L, Kun Z, Jie C, Yifang W, Yu Y, Jingfeng W et al (2013) Hemoglobin A1c can identify more cardiovascular and metabolic risk profile in OGTT-negative Chinese population. Int J Med Sci 10(8):1028–1034

    Article  CAS  Google Scholar 

  25. Tarasov KV, Sanna S, Scuteri A (2009) COL4A1 Is associated with arterial stiffness by genome-wide association scan. Circ Cardiovasc Genet 2:151–158

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  26. Naghshtabrizi B, Hajilooi M, Mozayanimonfared A, Homayounfar S, Shoshtari R (2012) Is there any association between CD14 polymorphism and coronary artery disease? Iran Heart J 13(3):6–10

    Google Scholar 

  27. Koenig W, Khuseyinova N, Hoffmann MM, Marz W, Frohlich M, Hoffmeister A (2002) CD14 C(-260)3T polymorphism, plasma levels of the soluble endotoxin receptor CD14, their association with chronic infections and risk of stable coronary artery disease. J Am College Cardiol 40:34–42

    Article  CAS  Google Scholar 

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Acknowledgements

We are thankful for the study that was supported by intramural Grant from the Era’s Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India.

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Authors and Affiliations

  1. Molecular Biology Lab, Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Lucknow, UP, 226025, India

    Syed Tasleem Raza, Shania Abbas, Ale Eba, Saliha Rizvi, Alina Zaidi & Farzana Mahdi

  2. Department of Cardiology, Era’s Lucknow Medical College and Hospital, Lucknow, India

    Fazal Karim & Irshad Ahmad Wani

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  1. Syed Tasleem Raza
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  2. Shania Abbas
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  3. Ale Eba
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  4. Fazal Karim
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Correspondence to Syed Tasleem Raza.

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Raza, S.T., Abbas, S., Eba, A. et al. Association of COL4A1 (rs605143, rs565470) and CD14 (rs2569190) genes polymorphism with coronary artery disease. Mol Cell Biochem 445, 117–122 (2018). https://doi.org/10.1007/s11010-017-3257-9

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  • DOI: https://doi.org/10.1007/s11010-017-3257-9

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