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Mir-513a-3p contributes to the controlling of cellular migration processes in the A549 lung tumor cells by modulating integrin β-8 expression

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Abstract

Lung tumors are a frequent type of cancer in humans and a leading cause of death, and the late diagnostic contributes to high mortality rates. New therapeutic strategies are needed, and the heptapeptide angiotensin-(1-7) [ang-(1-7)] demonstrated the ability to control cancer growth rates and migration in vitro and in vivo. However, the possible use of the heptapeptide in clinical trials demands deeper analyses to elucidate molecular mechanisms of its effect in the target cells. In this study, we investigated relevant elements that control pro-inflammatory environment and cellular migration, focusing in the post-transcription mechanism using lung tumor cell line. In our cellular model, the microRNA-513a-3p was identified as a novel element targeting ITG-β8, thereby controlling the protein level and its molecular function in the controlling of migration and pro-inflammatory environment. These findings provide useful information for future studies, using miR-513a-3p as an innovative molecular tool to control lung tumor cell migration, which will support more effective clinical treatment of the patients with the widely used chemotherapeutic agents, increasing survival rates.

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Acknowledgements

This study was supported by research grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (474060/2012-8), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—2014/21645-2; 2013/21186-5), and INCT-Nano-Biofarmacêutica. The authors report no conflict of interest.

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Authors and Affiliations

  1. Laboratório de Biologia Molecular, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Rio Claro, São Paulo, 13506-900, Brazil

    Marina Bonfogo da Silveira & Karen C. M. Moraes

  2. Instituto de Química, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Araraquara, São Paulo, Brazil

    Kelvin Furtado Lima

  3. Núcleo de Pesquisa em Biologia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil

    Andrea Renata da Silva

  4. Laboratório de Fisiologia, Departmento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Robson Augusto Souza dos Santos

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  1. Marina Bonfogo da Silveira
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  2. Kelvin Furtado Lima
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  3. Andrea Renata da Silva
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  4. Robson Augusto Souza dos Santos
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  5. Karen C. M. Moraes
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Correspondence to Karen C. M. Moraes.

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da Silveira, M.B., Lima, K.F., da Silva, A.R. et al. Mir-513a-3p contributes to the controlling of cellular migration processes in the A549 lung tumor cells by modulating integrin β-8 expression. Mol Cell Biochem 444, 43–52 (2018). https://doi.org/10.1007/s11010-017-3229-0

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