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Total flavone of Desmodium styracifolium relieved apoptosis and autophagy of COM-induced HK-2 cells by regulating KIM-1 via p38/MAPK pathway

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Abstract

The purpose of the study was to investigate the mechanism of total flavone of Desmodium styracifolium (TFDS) in regulating the formation of urinary calculi. Protein levels of KIM-1, LC3-II, p-p38 were measured by Western blot. The effect of different COM concentrations, different TFDS concentrations, SB203580 (specific inhibitor of p38/MAPK), and overexpression of KIM-1 on cell viability were detected by WST-1 assay. The apoptotic cells and FITC positive cells were detected by flow cytometry. HK-2 cell viability decreased with the increase of COM concentration, and the protein levels of KIM-1, LC3-II, and p-p38 increased with the time. Blocking the p38/MAPK pathway or co-cultured with TFDS inhibited the effects of COM on apoptosis and autophagy of HK-2 cells. In addition, blocking the p38/MAPK pathway inhibited the expression of KIM-1. In COM-induced cells, after treated with SB203580, overexpression of KIM-1 could reverse the protection effect of SB203580 on COM-induced cell damage and the inhibition of SB203580 on COM-induced excessive autophagy, suggesting p38/MAPK regulated KIM-1 to regulate COM-induced cell apoptosis and autophagy. Finally, we proved that TFDS inhibited p38/MAPK pathway. And the protection effect of COM-induced cell injury increased with the increase of TFDS concentration, and the adhesion between COM and cells decreased with the increase of TFDS concentration. With the increase of the concentration of TFDS, p38/MAPK pathway was gradually inhibited, and KIM-1 and autophagy related proteins were decreased. TFDS inhibited HK-2 cell apoptosis and autophagy by regulating KIM-1 via p38/MAPK pathway.

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Acknowledgements

This project was supported by research grants awarded by the Traditional Chinese medicine and Western Medicine Research of Tianjin health and Family Planning Commission (2015134).

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  1. Haijie Xie and Jie Li have contributed equally to this work.

Authors and Affiliations

  1. Urolithiasis Treatment Center of Tianjin, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, No. 23 Pingjiang Road, Hexi District, Tianjin, 300211, China

    Haijie Xie, Jie Li, Hongwei Gao, Jun Wang, Chuanbo Li, Yong Xu & Chunyu Liu

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  1. Haijie Xie
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  2. Jie Li
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  3. Hongwei Gao
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  4. Jun Wang
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  5. Chuanbo Li
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  6. Yong Xu
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  7. Chunyu Liu
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Correspondence to Yong Xu or Chunyu Liu.

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Xie, H., Li, J., Gao, H. et al. Total flavone of Desmodium styracifolium relieved apoptosis and autophagy of COM-induced HK-2 cells by regulating KIM-1 via p38/MAPK pathway. Mol Cell Biochem 442, 169–175 (2018). https://doi.org/10.1007/s11010-017-3201-z

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  • DOI: https://doi.org/10.1007/s11010-017-3201-z

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