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Molecular and Cellular Biochemistry

, Volume 442, Issue 1–2, pp 73–80 | Cite as

Resveratrol attenuates high glucose-induced endothelial cell apoptosis via mediation of store-operated calcium entry

  • Ting Lu
  • Dayan Zhou
  • Pan Gao
  • Liangyi Si
  • Qiang Xu
Article

Abstract

The aim of this study was to evaluate the influence of resveratrol on HG-induced calcium entry in islet microvascular (MS-1) endothelial cells. MS-1 cells were pretreated with resveratrol or 2-APB (an inhibitor of store-operated calcium entry) and then incubated with high glucose. Cell viability was determined using the cell counting kit-8 method. Reactive oxygen species, endothelial apoptosis, and NO production were detected by DHE probe, TUNEL detection, and nitrate reductase assay kit. Protein levels of SOCE were detected by western blotting. Pretreatment with resveratrol significantly attenuated HG-induced endothelial apoptosis and improved cell viability. However, pretreatment with resveratrol and 2-APB abolished this effect, suggesting that the attenuation of HG-induced apoptosis by resveratrol may be associated with SOCE. Subsequent analyses indicated that HG induced the SOCE-related proteins, including TRPC1, Orai1, and Stim1. These results suggest that resveratrol pretreatment is associated with relieved HG-induced endothelial apoptosis at least partly via inhibition of SOCE-related proteins.

Keywords

Resveratrol Store-operated calcium entry (SOCE) Endothelial cells Apoptosis High glucose 

Notes

Acknowledgements

We thank Fang LI for technique assistance in measuring intracellular Ca2+. The study was supported by Chongqing characteristics of specialist construction funds.

Authors’ contributions

Qiang Xu conceived of the study. Ting Lu carried out cell culture and Western blotting analysis, participated in TUNEL staining, and measurement of intracellular Ca2+. Dayan Zhou performed the statistical analysis. Pan Gao drafted the manuscript. Liangyi Si participated in the design of the study. All authors contributed to and have approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

11010_2017_3194_MOESM1_ESM.tif (12.5 mb)
Supplemental Fig. 1 Scheme image of the signaling mechanisms underlying inhibitory effect of resveratrol for high glucose-induced endothelial cell apoptosis via mediation of store-operated calcium entry. Calcium ion receptor (Stim1), which locates in the endoplasmic reticulum, was found to form Ca2+ channel with Orai1 when the TG was induced to release Ca2+ from the endoplasmic reticulum. High-glucose stimulates apoptosis of endothelial cells via induction of calcium influx through the activation of the SOC channel. Resveratrol functions via inhibiting the activation of the SOC channel to reduce calcium influx, and also via reducing the high glucose-induced reactive oxygen species generation, thereby reducing endothelial cell apoptosis. Supplementary material 1 (TIFF 12837 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Ting Lu
    • 1
  • Dayan Zhou
    • 1
  • Pan Gao
    • 2
  • Liangyi Si
    • 3
  • Qiang Xu
    • 1
  1. 1.Department of CardiologyChongqing Fifth People ‘s HospitalChongqingChina
  2. 2.Department of GeriatricsThe First Affiliated Hospital of the Third Military Medical UniversityChongqingChina
  3. 3.Chongqing Medical University, Third Affiliated HospitalChongqingChina

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