Molecular and Cellular Biochemistry

, Volume 442, Issue 1–2, pp 19–28 | Cite as

The CCAAT box in the proximal SERCA2 gene promoter regulates basal and stress-induced transcription in cardiomyocytes

  • Jorge Fragoso-Medina
  • Gabriela Rodriguez
  • Angel Zarain-Herzberg


The cardiac sarco/endoplasmic reticulum Ca2+-ATPase-2a (SERCA2a) is vital for the correct handling of calcium concentration in cardiomyocytes. Recent studies showed that the induction of endoplasmic reticulum (ER) stress (ERS) with the SERCA2 inhibitor Thapsigargin (Tg) increases the mRNA and protein levels of SERCA2a. The SERCA2 gene promoter contains an ERS response element (ERSE) at position −78 bp that is conserved among species and might transcriptionally regulate SERCA2 gene expression. However, its involvement in SERCA2 basal and calcium-mediated transcriptional activation has not been elucidated. In this work, we show that in cellular cultures of neonatal rat ventricular myocytes, the treatment with Tg or the calcium ionophore A23187 increases the SERCA2a mRNA and protein abundance, as well as the transcriptional activity of two chimeric human SERCA2 gene constructs, containing −254 and −2579 bp of 5′-regulatory region cloned in the pGL3-basic vector and transiently transfected in cultured cardiomyocytes. We found that the ERSE present in the SERCA2 proximal promoter contains a CCAAT box that is involved in basal and ERS-mediated hSERCA2 transcriptional activation. The EMSA results showed that the CCAAT box present in the ERSE recruits the NF-Y transcription factor. Additionally, by ChIP assays, we confirmed in vivo binding of NF-Y and C/EBPβ transcription factors to the SERCA2 gene proximal promoter.


SERCA2 Stress Calcium Transcriptional regulation NF-Y C/EBPβ 



The work was funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) Grant 164413 to A.Z.-H. and by Scholarship CONACyT 271055 to J.F.-M. We thank Dr. Iván Meneses-Morales, Dr. Rafael Estrada-Avilés, and M.Sc. Eduardo Izquierdo-Torres for their valuable comments to improve this manuscript.


None of the authors have financial or personal relationships that could influence their work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Supplementary material

11010_2017_3189_MOESM1_ESM.pdf (196 kb)
Supplementary material 1 (PDF 195 kb)
11010_2017_3189_MOESM2_ESM.pdf (208 kb)
Supplementary material 2 (PDF 207 kb)


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Departamento de Bioquímica, Facultad de Medicina, School of MedicineUniversidad Nacional Autónoma de MéxicoMexico CityMexico

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