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Molecular and Cellular Biochemistry

, Volume 428, Issue 1–2, pp 193–202 | Cite as

Expression and functional activity of bitter taste receptors in primary renal tubular epithelial cells and M-1 cells

Article

Abstract

The kidney is essential in the maintenance of in vivo homeostasis by body fluid and electrolyte conservation and metabolic waste removal. Previously, we reported the expression of a novel G protein family (Tas2rs), which includes bitter taste receptors, in the kidney tubule system, including the nephrons and the collecting duct system. Bitter taste receptors could affect kidney function via Ca2+ intake. Alkaloids such as phenylthiocarbamide stimulate these receptors and cause an increase in Ca2+ intake. In this study, we determined the expression of bitter taste receptors in the immature kidney and small intestine and in primary renal epithelial cells and M-1 (collecting tubule cell line) cells, by using QPCR and immunostaining. We found no expression of bitter taste receptors in the immature kidney and small intestine several days after birth; the relative abundance of Tas2rs transcripts varied depending on the developmental stage. Tas2rs were expressed in primary renal epithelial cells and M-1 cells. The traditional Chinese medicinal plant extracts phellodendrine and coptisine caused a rapid rise in intracellular Ca2+ concentration, which was inhibited by the phospholipase C (PLC) inhibitor U-73122. Thus, phellodendrine and coptisine could change the physiological status of renal cells in vitro by mediation of bitter taste receptors in a PLC-dependent manner. Our results provide new insights on the expression and role of bitter taste receptors in renal development and function.

Keywords

Kidney Bitter taste receptors Primary renal tubular epithelial cells M-1 cells Alkaloid 

Notes

Acknowledgements

The present study was supported by the Chinese National Natural Science Foundation (Grant Nos. 31571171 and 31100838), the Shanghai Natural Science Foundation (Grant No. 15ZR1414900), the Key Laboratory of Medical Electrophysiology (Southwest Medical University) of Ministry of Education of China (Grant No. 201502), and the Young Teachers of Shanghai Universities Training Program.

Supplementary material

11010_2016_2929_MOESM1_ESM.tif (238 kb)
Supplementary material 1 (TIF 237 KB)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.School of Life SciencesShanghai UniversityShanghaiPeople’s Republic of China
  2. 2.Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, Shanghai Medical CollegeFudan UniversityShanghaiChina

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