Molecular and Cellular Biochemistry

, Volume 428, Issue 1–2, pp 79–86 | Cite as

The hedgehog-signaling pathway is repressed during the osteogenic differentiation of dental follicle cells

  • C. Morsczeck
  • A. Reck
  • H. C. Beck


Dental follicle stem cells (DFCs) are precursor cells of alveolar osteoblasts, and previous studies have shown that the growth factor bone morphogenetic protein (BMP)2 induces the osteogenic differentiation of DFCs. However, the molecular mechanism down-stream of the induction of the osteogenic differentiation by BMP2 remains elusive. We investigated therefore the phosphoproteome of DFCs after the induction of the osteogenic differentiation with BMP2. In this study, phosphoproteins of the hedgehog “off” state were differentially expressed. Further analyses revealed that BMP2 induced the expression of repressors of the hedgehog-signaling pathway such as Patched 1 (PTCH1), Suppressor of Fused (SUFU), and Parathyroid Hormone-Related Peptide (PTHrP). Previous studies suggested that hedgehog proteins induce the osteogenic differentiation of mesenchymal stem cells via a paracrine pathway. Indian hedgehog (IHH) induced the expression of the osteogenic transcription factor RUNX2. However, a supplementation of the BMP2-based osteogenic differentiation medium with IHH did not induce the expression of RUNX2. Moreover, IHH inhibited slightly the ALP activity and the mineralization of osteogenic-differentiated DFCs. In conclusion, our results suggest that BMP2 inhibits the hedgehog signaling after the induction of the osteogenic differentiation in DFCs.


Hedgehog-signaling pathway Dental follicle cells Osteogenic differentiation Periodontium Bone morphogenetic protein 

Supplementary material

11010_2016_2918_MOESM1_ESM.pdf (713 kb)
Supplementary material 1 (PDF 712 KB)


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Cranio- and Maxillofacial SurgeryHospital of the University of RegensburgRegensburgGermany
  2. 2.Department of Clinical Biochemistry and Pharmacology, Centre for Clinical ProteomicsOdense University HospitalOdenseDenmark

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