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Molecular and Cellular Biochemistry

, Volume 428, Issue 1–2, pp 1–8 | Cite as

Regulation of PPARγ and CIDEC expression by adenovirus 36 in adipocyte differentiation

  • Yi Jiao
  • Yiliyasi Aisa
  • Xiaodi Liang
  • Nuerbiye Nuermaimaiti
  • Xian Gong
  • Zhaoxia Zhang
  • Yaqun Guan
Article

Abstract

This study is to investigate the role of adenovirus 36 (Ad36) in regulating expression of peroxisome proliferator-activated receptor γ (PPARγ) and cell death-inducing DFFA-like effector c (CIDEC) in Ad36-induced adipocyte differentiation. Human adipose-derived mesenchymal stem cells (hAMSCs) were isolated and cultured, and then infected with Ad36. Ad36-induced adipocytes were identified using quantitative real-time PCR and Oil red O staining. The expression levels of PPARγ and CIDEC in Ad36-induced adipocytes were determined by quantitative real-time PCR and Western blot analysis. Glucose uptake and intracellular triglyceride content were also determined in these induced cells. Our results from the Oil red O staining showed that Ad36 induced the differentiation of hAMSCs into human adipocytes in vitro. Moreover, the medium glucose concentration was significantly decreased, while the intracellular triglyceride content was significantly increased, in the Ad36-induced adipocytes, compared with the control group. Furthermore, our results showed that, the mRNA and protein expression levels of PPARγ and CIDEC were significantly upregulated in Ad36-induced adipocytes, in a time-dependent manner. On the other hand, compared with the control group, the CIDEC expression was downregulated when the Ad36-induced adipocytes were treated with the PPARγ inhibitor, GW9662. Ad36 could upregulate the expression level of CIDEC through increasing PPARγ expression during the adipocyte differentiation process.

Keywords

Human adipose-derived mesenchymal stem cell (hAMSCs) Adenovirus 36 (Ad36) PPARγ CIDEC 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 81660154) and the College Students Innovation Experiment Program (CX2015002).

Compliance with ethical standards

Conflict of interest

All authors declare no financial conflict of interest. All authors declare no non-financial conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yi Jiao
    • 1
  • Yiliyasi Aisa
    • 1
  • Xiaodi Liang
    • 1
  • Nuerbiye Nuermaimaiti
    • 1
  • Xian Gong
    • 1
  • Zhaoxia Zhang
    • 2
  • Yaqun Guan
    • 1
    • 3
  1. 1.Department of Biochemistry, Preclinical Medicine CollegeXinjiang Medical UniversityÜrümqiChina
  2. 2.Medical Testing CenterThe First Affiliated Hospital of Xinjiang Medical UniversityÜrümqiChina
  3. 3.Diabetes CenterThe First Affiliated Hospital of Xinjiang Medical UniversityÜrümqiChina

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