Molecular and Cellular Biochemistry

, Volume 416, Issue 1–2, pp 117–129 | Cite as

Evaluation of the effect of n-3 PUFA-rich dietary fish oils on lipid profile and membrane fluidity in alloxan-induced diabetic mice (Mus musculus)

  • Shantal Ganapati Kamat
  • Ramaballav Roy


Marine fishes are important to health due to their high content of polyunsaturated fatty acids particularly those of the omega-3 family. These fatty acids play an important role in various physiological processes and as a consequence they may modulate and even prevent some human diseases. The aim of the present study was to investigate and compare the effect of fish oils of different origins (Sardinella longiceps, Rastrelliger kanagurta and Clarias batrachus) on lipid metabolism and membrane fluidity in diabetes. Alloxan was injected in repetitive doses for 1 month (100 mg/kg body weight every 5th day) to induce diabetes in Swiss albino mice. 10 % S. longiceps, R. kanagurta or C. batrachus fish oil was freshly blended with pellet feed which was provided to diabetic mice for 1 month. The serum lipid profile (serum total cholesterol, triglyceride, HDL, VLDL and LDL) along with liver, kidney and heart tissue lipid profile (i.e. triglyceride, total cholesterol, glycolipid and phospholipid) was analysed. Besides, the enzymatic activity of HMG-CoA reductase, HMG-CoA synthase and glucose-6-phosphate-dehydrogenase along with the membrane fluidity of these tissues was evaluated. Altered tissue lipid composition, enzyme activities and membrane fluidity due to diabetes were returned towards normal with the supplementation of 10 % fish oils. Fish oil from S. longiceps brought maximum changes in level of neutral lipid composition in heart, and increased the concentration of phospholipid and decreased the activity of HMG-CoA reductase in comparison with the fish oil from R. kanagurta and C. batrachus.


Diabetes Fish oil Lipid Membrane fluidity PUFA Insulin 



High-density lipoprotein cholesterol


Very low-density lipoprotein cholesterol


Low-density lipoprotein cholesterol








Free fatty acid




Total cholesterol


Cholesteryl esters

HMG-CoA reductase

3-Hydroxy-3-methyl-glutaryl-CoA reductase

HMG-CoA synthase

3-Hydroxy-3-methyl-glutaryl-CoA synthase




Eicosapentaenoic acid


Docosahexaenoic acid


Polyunsaturated fatty acid


Diabetes mellitus



Financial support from the University Grant Commission-Special Assistance Programme India (Sanction letter no. F.3-3/2011 (SAPII) is acknowledged. We thank Professor Philip Calder, University of Southampton, for assistance with writing and for comments that greatly improved the manuscript.

Compliance with ethical standards

Conflict of interest

The study was carried out in strict accordance as per the guidelines of CPCSEA (Committee for the Purpose of Control and Supervision on Experiments on Animals, Govt. of India). The authors declare that they have no conflicts of interest concerning this article.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of ZoologyGoa UniversityTaleigao PlateauIndia

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