Molecular and Cellular Biochemistry

, Volume 413, Issue 1–2, pp 119–125 | Cite as

Color reduction of melanin by lysosomal and peroxisomal enzymes isolated from mammalian cells

  • Dong Jun Park
  • Simranjeet Singh Sekhon
  • Jihee Yoon
  • Yang-Hoon Kim
  • Jiho Min


Lysosomes and peroxisomes are organelles with many functions in all eukaryotic cells. Lysosomes contain hydrolytic enzymes (lysozyme) that degrade molecules, whereas peroxisomes contain enzymes such as catalase that convert hydrogen peroxide (H2O2) to water and oxygen and neutralize toxicity. In contrast, melanin is known as a helpful element to protect the skin against harmful ultraviolet rays. However, a high quantity of melanin leads to hyperpigmentation or skin cancer in human. New materials have already been discovered to inhibit tyrosinase in melanogenesis; however, melanin reduction does not suggest their preparation. In this study, we report that the color intensity because of melanin decreased by the cellular activation of lysosomes and peroxisomes. An increase in the superficial intensity of lysosome and peroxisome activities of HeLa cells was observed. In addition, a decrease in the amount of melanin has also been observed in mammalian cells without using any other chemical, showing that the process can work in vivo for treating melanin. Therefore, the results of this study indicate that the amount of melanin decreases by the lysosome and peroxisome activity after entering the cells, and functional organelles are effective in color reduction. This mechanism can be used in vivo for treating melanin.


Lysosomes Peroxisomes Melanin Reactive oxygen species (ROS) Degradation 



This work was carried out with the support of “Cooperative Research Program for agriculture Science & Technology Development (Project title: Extraction and utilization technology development of functional materials in poultry egg white, Project No:PJ01164101)” Rural Development Administration, Republic of Korea. The authors are grateful for their support.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Bioprocess EngineeringChonbuk National UniversityJeonjuSouth Korea
  2. 2.Department of MicrobiologyChungbuk National UniversityCheongjuSouth Korea
  3. 3.Division of Chemical EngineeringChonbuk National UniversityJeonjuSouth Korea

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