Color reduction of melanin by lysosomal and peroxisomal enzymes isolated from mammalian cells
Lysosomes and peroxisomes are organelles with many functions in all eukaryotic cells. Lysosomes contain hydrolytic enzymes (lysozyme) that degrade molecules, whereas peroxisomes contain enzymes such as catalase that convert hydrogen peroxide (H2O2) to water and oxygen and neutralize toxicity. In contrast, melanin is known as a helpful element to protect the skin against harmful ultraviolet rays. However, a high quantity of melanin leads to hyperpigmentation or skin cancer in human. New materials have already been discovered to inhibit tyrosinase in melanogenesis; however, melanin reduction does not suggest their preparation. In this study, we report that the color intensity because of melanin decreased by the cellular activation of lysosomes and peroxisomes. An increase in the superficial intensity of lysosome and peroxisome activities of HeLa cells was observed. In addition, a decrease in the amount of melanin has also been observed in mammalian cells without using any other chemical, showing that the process can work in vivo for treating melanin. Therefore, the results of this study indicate that the amount of melanin decreases by the lysosome and peroxisome activity after entering the cells, and functional organelles are effective in color reduction. This mechanism can be used in vivo for treating melanin.
KeywordsLysosomes Peroxisomes Melanin Reactive oxygen species (ROS) Degradation
This work was carried out with the support of “Cooperative Research Program for agriculture Science & Technology Development (Project title: Extraction and utilization technology development of functional materials in poultry egg white, Project No:PJ01164101)” Rural Development Administration, Republic of Korea. The authors are grateful for their support.
- 1.Claudine T, Patrice C, Helene A, Thierry L, Nathalie A-A (2006) Lysosomes and lysosomal proteins in cancer cell death. Biochim Biophys Acta 1765:101–125Google Scholar
- 6.Fawcett DW (1966) The cell: peroxisomes. W.B. Saunders Company, PhiladelphiaGoogle Scholar
- 15.Suzukawa AA, Vieira A, Winnischofer SMB, Scalfo AC, Mascio PD, Costa Ferreira AM, Ravanat JL, Luna Martins D, Rocha MEM, Martinez GR (2012) Novel properties of melanins include promotion of DNA strand breaks, impairment of repair, and reduced ability to damage DNA after quenching of singlet oxygen. Free Radic Biol Med 52:1945–1953CrossRefPubMedGoogle Scholar
- 24.Mukherjee PK, Badami S, Wahile AM, Rajan S, Suresh B (2001) Evaluation of tyrosinase inhibitory activity of some indian spices. J Nat Remedies 1(2):125–129Google Scholar
- 26.XiFeng L, Yong Li, Jeong JH, Lee KT, Choi HD, Son BW (2003) Screening of tyrosinase inhibiting activity from the marine-derived fungus. Korean J Pharmacogn 34(2):138–141Google Scholar