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Molecular and Cellular Biochemistry

, Volume 413, Issue 1–2, pp 119–125 | Cite as

Color reduction of melanin by lysosomal and peroxisomal enzymes isolated from mammalian cells

  • Dong Jun Park
  • Simranjeet Singh Sekhon
  • Jihee Yoon
  • Yang-Hoon Kim
  • Jiho Min
Article

Abstract

Lysosomes and peroxisomes are organelles with many functions in all eukaryotic cells. Lysosomes contain hydrolytic enzymes (lysozyme) that degrade molecules, whereas peroxisomes contain enzymes such as catalase that convert hydrogen peroxide (H2O2) to water and oxygen and neutralize toxicity. In contrast, melanin is known as a helpful element to protect the skin against harmful ultraviolet rays. However, a high quantity of melanin leads to hyperpigmentation or skin cancer in human. New materials have already been discovered to inhibit tyrosinase in melanogenesis; however, melanin reduction does not suggest their preparation. In this study, we report that the color intensity because of melanin decreased by the cellular activation of lysosomes and peroxisomes. An increase in the superficial intensity of lysosome and peroxisome activities of HeLa cells was observed. In addition, a decrease in the amount of melanin has also been observed in mammalian cells without using any other chemical, showing that the process can work in vivo for treating melanin. Therefore, the results of this study indicate that the amount of melanin decreases by the lysosome and peroxisome activity after entering the cells, and functional organelles are effective in color reduction. This mechanism can be used in vivo for treating melanin.

Keywords

Lysosomes Peroxisomes Melanin Reactive oxygen species (ROS) Degradation 

Notes

Acknowledgements

This work was carried out with the support of “Cooperative Research Program for agriculture Science & Technology Development (Project title: Extraction and utilization technology development of functional materials in poultry egg white, Project No:PJ01164101)” Rural Development Administration, Republic of Korea. The authors are grateful for their support.

References

  1. 1.
    Claudine T, Patrice C, Helene A, Thierry L, Nathalie A-A (2006) Lysosomes and lysosomal proteins in cancer cell death. Biochim Biophys Acta 1765:101–125Google Scholar
  2. 2.
    Ohtaki N, Seiji M (1971) Degradation of melanosomes by lysosomes. J Investig Dermatol 57(1):1–5CrossRefGoogle Scholar
  3. 3.
    Yoon J, Park JMP, Kim KJ, Kim YH, Min J (2009) Antimicrobial activity of the cell organelles, lysosomes, isolated from egg white. J Microbiol Biotechnol 19(11):1364–1368CrossRefPubMedGoogle Scholar
  4. 4.
    Yoon J, Park JM, Jung SK, Kim KY, Kim YH, Min J (2009) Characterization of antimicrobial activity of the lysosomes isolated from Saccharomyces cerevisiae. Curr Microbiol 59:48–52CrossRefPubMedGoogle Scholar
  5. 5.
    Gallis HA, Miller SE, Wheat RW (1976) Degradation of 14C-labeled streptococcal cell walls by egg white lysozyme and lysosomal enzymes. Infect Immun 13(5):1459–1466PubMedCentralPubMedGoogle Scholar
  6. 6.
    Fawcett DW (1966) The cell: peroxisomes. W.B. Saunders Company, PhiladelphiaGoogle Scholar
  7. 7.
    Kunau WH (1998) Peroxisome biogenesis: from yeast to man. Curr Opin Microbiol 1:232–237CrossRefPubMedGoogle Scholar
  8. 8.
    Fidaleo M (2010) Peroxisomes and peroxisomal disorders: the main facts. Exp Toxicol Pathol 62:615–625CrossRefPubMedGoogle Scholar
  9. 9.
    Schrader M, Dariush FH (2004) Mammalian peroxisomes and reactive oxygen stress. Histochem Cell Biol 122:383–393CrossRefPubMedGoogle Scholar
  10. 10.
    Schrader M, Fahimi HD (2006) Peroxisomes and oxidative stress. Biochim Biophys 1763:1755–1766CrossRefGoogle Scholar
  11. 11.
    Marcol V, Beermann F (1996) Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett 381:165–168CrossRefGoogle Scholar
  12. 12.
    Slominski A, Tobin DJ, Shibahara S, Wortsman J (2004) Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev 84:1155–1228CrossRefPubMedGoogle Scholar
  13. 13.
    Riley PA (2003) Melanogenesis and melanoma. Pigment Cell Res 16:548–552CrossRefPubMedGoogle Scholar
  14. 14.
    Scherer D, Kumar R (2010) Genetics of pigmentation in skin cancer. Mutat Res 705:141–153CrossRefPubMedGoogle Scholar
  15. 15.
    Suzukawa AA, Vieira A, Winnischofer SMB, Scalfo AC, Mascio PD, Costa Ferreira AM, Ravanat JL, Luna Martins D, Rocha MEM, Martinez GR (2012) Novel properties of melanins include promotion of DNA strand breaks, impairment of repair, and reduced ability to damage DNA after quenching of singlet oxygen. Free Radic Biol Med 52:1945–1953CrossRefPubMedGoogle Scholar
  16. 16.
    Hill HZ, Hill GJ (1987) Eumelanin causes DNA strand breaks and kills cells. Pigment Cell Res 1:163–170CrossRefPubMedGoogle Scholar
  17. 17.
    Borovansky J, Mommassa AM, Smit NPM, Eygendaal D, Winder AJ, Vermeer BJ, Pavel S (1997) Melanogenesis in transfected fibroblasts induces lysosomal activation. Arch Dermatol Res 289:145–150CrossRefPubMedGoogle Scholar
  18. 18.
    Yoon J, Bang SH, Park JS, Chang ST, Kim YH, Min J (2011) Increased in vitro lysosomal function in oxidative stress-induced cell lines. Appl Biochem Biotechnol 163:1002–1011CrossRefPubMedGoogle Scholar
  19. 19.
    Chan YY, Kim KH, Cheah SH (2011) Inhibitory effects of sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. J Ethnopharmacol 137:1183–1188CrossRefPubMedGoogle Scholar
  20. 20.
    Momtaz S, Lall N, Basson A (2008) Inhibitory activities of mushroom tyrosine and DOPA oxidation by plant extracts. S Afr J Bot 74:577–582CrossRefGoogle Scholar
  21. 21.
    Curto EV, Kwong C, Hermersdörfer H, Glatt H, Santis C, Virador V, Hearing VJ, Dooley TP (1999) Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochem Pharmacol 57:663–672CrossRefPubMedGoogle Scholar
  22. 22.
    Winder AJ, Harris H (1991) New assays for the tyrosine hydroxylase and dopa oxidase activities of tyrosinase. Eur J Biochem 198:317–326CrossRefPubMedGoogle Scholar
  23. 23.
    Pajak S, Hopwood LE, Hyde JS, Felix CC, Sealy RC, Kushnaryov VM, Hatchell MC (1983) Melanin Endocytosis by cultured mammalian cells. Exp Cell Res 149:513–526CrossRefPubMedGoogle Scholar
  24. 24.
    Mukherjee PK, Badami S, Wahile AM, Rajan S, Suresh B (2001) Evaluation of tyrosinase inhibitory activity of some indian spices. J Nat Remedies 1(2):125–129Google Scholar
  25. 25.
    Moon JY, Yim EY, Song G, Lee NH, Hyun CG (2010) Screening of elastase and tyrosinase inhibitory activity from Jeju island plants. Eur Asia J Biosci 4:41–53CrossRefGoogle Scholar
  26. 26.
    XiFeng L, Yong Li, Jeong JH, Lee KT, Choi HD, Son BW (2003) Screening of tyrosinase inhibiting activity from the marine-derived fungus. Korean J Pharmacogn 34(2):138–141Google Scholar
  27. 27.
    Kokkinakis DM, Brooks JL (1979) Tomato peroxidase: purification, characterization, and catalytic properties. Plant Physiol 63(1):93–99PubMedCentralCrossRefPubMedGoogle Scholar
  28. 28.
    Pettigrew GW, Seilman S (1982) Purification and properties of a cross linked complex between cytochrome c and cytochrome c peroxidase. Biochem J 201(1):9–18PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Bioprocess EngineeringChonbuk National UniversityJeonjuSouth Korea
  2. 2.Department of MicrobiologyChungbuk National UniversityCheongjuSouth Korea
  3. 3.Division of Chemical EngineeringChonbuk National UniversityJeonjuSouth Korea

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