Molecular and Cellular Biochemistry

, Volume 412, Issue 1–2, pp 221–227 | Cite as

Coronary flow and oxidative stress during local anaphylactic reaction in isolated mice heart: the role of nitric oxide (NO)

  • Vesna Milicic
  • Vladimir Zivkovic
  • Nevena Jeremic
  • Nebojsa Arsenijevic
  • Dragan Djuric
  • Vladimir Lj. Jakovljevic


The aim of this study was to assess the role of nitric oxide (NO) in cardiac anaphylaxis regarding changes in coronary reactivity and oxidative status of the mice heart. The animals were divided into two groups: experimental group (CBA, iNOS−/− mice) and control group: wild-type mice (CBA/H). The hearts of male mice (n = 24; 6–8 weeks old, body mass 20–25 g, 12 in each experimental group) were excised and retrogradely perfused according to the Langendorff technique at a constant perfusion pressure (70 cm H2O). Cardiac anaphylaxis was elicited by injection of solution (1 mg/1 ml) of ovalbumin into the aortic cannula. For the next 10 min, in intervals of 2 min (0–2, 2–4, 4–6, 6–8, 8–10 min) coronary flow (CF) rates were measured and samples of coronary effluent were collected. Markers of oxidative stress including index of lipid peroxidation measured as thiobarbituric acid-reactive substances (TBARS), NO measured in the form of nitrites (\({\text{NO}}_{2}^{ - }\)), superoxide anion radical (\({\text{O}}_{2}^{ - }\)), and hydrogen peroxide (H2O2) in the coronary venous effluent were assessed spectrophotometrically. After the ovalbumin challenge, CF was significantly lower in the wild mice group. NO and H2O2 release were significantly higher in iNOS−/− mice group. TBARS and \({\text{O}}_{2}^{ - }\) values did not vary significantly between wild and iNOS−/− mice groups. Our results indicate that coronary vasoconstriction during cardiac anaphylaxis does not necessarily depend on inducible nitric oxide synthase (iNOS)/NO activity and that iNOS/NO pathway may not be an only influential mediator of redox changes in this model of cardiac anaphylaxis.


Cardiac anaphylaxis Isolated mice heart Coronary flow Nitric oxide Oxidative stress 



Coronary flow


Endothelial nitric oxide synthase


Peroxidase from horse radish


Inducible nitric oxide synthase


Immunoglobulin E


Nitro blue tetrazolium


Nitric oxide


Phenol red solution


Platelet-activating factor


Reactive oxygen species


Thiobarbituric acid-reactive substances


Thiobarbituric acid



This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 175043), and the Faculty of Medical Sciences, University of Kragujevac (Junior Project 04/11).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interests relevant to the manuscript.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Vesna Milicic
    • 1
  • Vladimir Zivkovic
    • 2
  • Nevena Jeremic
    • 3
  • Nebojsa Arsenijevic
    • 4
  • Dragan Djuric
    • 5
  • Vladimir Lj. Jakovljevic
    • 2
  1. 1.Department of Dermatovenerology, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  2. 2.Department of Physiology, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  3. 3.Department of Pharmacy, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  4. 4.Center for Molecular Medicine, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  5. 5.Institute of Medical Physiology “Richard Burian”, Faculty of MedicineUniversity of BelgradeBelgradeSerbia

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