Molecular and Cellular Biochemistry

, Volume 412, Issue 1–2, pp 209–219 | Cite as

Detraining reverses exercise-induced improvement in blood pressure associated with decrements of oxidative stress in various tissues in spontaneously hypertensive rats

  • Ozgen Kilic-Erkek
  • Emine Kilic-Toprak
  • Sadettin Caliskan
  • Yusuf Ekbic
  • Ismail Hakki Akbudak
  • Vural Kucukatay
  • Melek Bor-Kucukatay


This study aimed to investigate the effects of moderate intensity swimming exercise (10 weeks) followed by detraining (for five and 10 weeks) on oxidative stress levels of heart, lung, kidney, and liver tissues and systolic blood pressure (SBP) of spontaneously hypertensive rats (SHR). SHR and control rats were randomized into sedentary, exercised, detrained (5 weeks) and late-detrained (10 weeks) groups. Corresponding sedentary rats were grouped as time 1-2-3. Exercise of 60 min, 5 days/week/10 weeks was applied. Detraining rats underwent the same training protocol and then discontinued training during next 5, 10 weeks. SBP was measured by tail-cuff method. Tissue total oxidant/antioxidant status was measured using a commercial kit and oxidative stress index (OSI) was calculated. Exercise training slightly decreased tissue OSI of SHR and reduced SBP of both groups. Tissue OSI of SHR were higher than WKY and aging resulted in increment of oxidants in groups. detraining yielded time-dependent increments in oxidative stress of all tissues and SBP of both rat groups. Although short-term cessations may be tolerated, our results emphasize the importance of exercising as a way of life for cardiovascular well-being in hypertensives or in individuals who are genetically under risk of hypertension.


Swimming Physical inactivity Hypertension Total oxidant-antioxidant status Tissue oxidative stress 



This study was supported by Pamukkale University Scientific Research Projects Coordination Unit through project numbers 2012ARŞ002, 2013SBE002, 2013SBE003, and 2015HZL010.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ozgen Kilic-Erkek
    • 1
  • Emine Kilic-Toprak
    • 2
  • Sadettin Caliskan
    • 1
  • Yusuf Ekbic
    • 1
  • Ismail Hakki Akbudak
    • 3
  • Vural Kucukatay
    • 1
  • Melek Bor-Kucukatay
    • 1
  1. 1.Faculty of Medicine, Department of PhysiologyPamukkale UniversityDenizliTurkey
  2. 2.Denizli Health Services Vocational High School, Department of PhysiotherapyPamukkale UniversityDenizliTurkey
  3. 3.Faculty of Medicine, Department of Internal MedicineErciyes UniversityKayseriTurkey

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