Molecular and Cellular Biochemistry

, Volume 401, Issue 1–2, pp 133–139 | Cite as

Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus

  • Martin Ošt’ádal
  • Adam Eckhardt
  • Jan Herget
  • Ivan Mikšík
  • Pavel Dungl
  • Jiří Chomiak
  • Monika Frydrychová
  • Michal Burian


Idiopathic pes equinovarus is a congenital deformity of the foot and lower leg defined as a fixation of the foot in adduction, supination, and varus. Although the pathogenesis of clubfoot remains unclear, it has been suggested that fibroblasts and growth factors are involved. To directly analyze the protein composition of the extracellular matrix in contracted tissue of patients with clubfoot. A total of 13 infants with idiopathic clubfoot treated with the Ponseti method were included in the present study. Tissue samples were obtained from patients undergoing surgery for relapsed clubfeet. Contracted tissues were obtained from the medial aspect of the talonavicular joint. Protein was extracted after digestion and delipidation using zip-tip C18. Individual collagenous fractions were detected using a chemiluminescent assay. Amino acid analysis of tissue samples revealed a predominance of collagens, namely collagen types I, III, and VI. The high content of glycine and h-proline suggests a predominance of collagens I and III. A total of 19 extracellular matrix proteins were identified. The major result of the present study was the observation that the extracellular matrix in clubfoot is composed of an additional 16 proteins, including collagens V, VI, and XII, as well as the previously described collagen types I and III and transforming growth factor β. The characterization of the general protein composition of the extracellular matrix in various regions of clubfoot may help in understanding the pathogenesis of this anomaly and, thus, contribute to the development of more efficacious therapeutic approaches.


Collagens Extracellular matrix Pes equinovarus Proteomics 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Martin Ošt’ádal
    • 1
  • Adam Eckhardt
    • 2
  • Jan Herget
    • 3
  • Ivan Mikšík
    • 2
  • Pavel Dungl
    • 1
  • Jiří Chomiak
    • 1
  • Monika Frydrychová
    • 1
  • Michal Burian
    • 1
  1. 1.Department of Orthopaedics, 1st Faculty of Medicine, University Hospital BulovkaCharles UniversityPrague 8Czech Republic
  2. 2.Institute of PhysiologyAcademy of Sciences of the Czech RepublicPrague 4Czech Republic
  3. 3.Institute of Physiology, 2nd Faculty of MedicineCharles UniversityPrague 5Czech Republic

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