Molecular and Cellular Biochemistry

, Volume 387, Issue 1–2, pp 187–195 | Cite as

Upregulated Parkin expression protects mitochondrial homeostasis in DJ-1 konckdown cells and cells overexpressing the DJ-1 L166P mutation



Rare genetic mutations in the DJ-1 and Parkin genes cause recessive Parkinsonism, however, the relationship between these two genes is not fully elucidated. Current emerging evidence suggests that these genes are involved in mitochondrial homeostasis, and that a deficiency in either of these two genes is associated with damages in mitochondrial function and morphology. In this study, we demonstrated that knockdown of DJ-1 expression or the overexpression of the DJ-1 L166P mutation results in a damaged phenotype in mitochondria and a hypersensitivity to H2O2-induced cell apoptosis. These phenotypes result from increased levels of endogenous oxidative stress. However, overexpression of wild-type Parkin rescued the phenotypes observed in the mitochondria of DJ-1 knockdown and DJ-1 L166P mutant cells. We also determined that there were differences between the two cell models. Furthermore, both H2O2 treatment and the DJ-1 L166P mutation weakened the interaction between DJ-1 and Parkin. Taken together, these findings suggested that DJ-1 and Parkin were linked through oxidative stress, and that overexpression of Parkin protects DJ-1 protein-deficient and DJ-1 L166P mutant-expressing cells via inhibition of oxidative stress.


Parkinson’s disease Parkin DJ-1 Oxidative dysfunction Mitochondrial homeostasis 



This study was supported by grants obtained from the Doctoral Program of Higher Education of China (Grant No. 20100071110036), the Shanghai Municipal Natural Science Foundation, China (Grant No. 10ZR1403200), and Basic Research Project of Shanghai Science and Technology Commission (13JC1401101).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Cellular and Genetic MedicineSchool of Basic Medical Sciences Fudan UniversityShanghaiPeople’s Republic of China

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