Molecular and Cellular Biochemistry

, Volume 383, Issue 1–2, pp 173–177 | Cite as

Ethionine regulates cell motile activity through LPA receptor-3 in liver epithelial WB-F344 cells

  • Serina Inoue
  • Eriko Tanabe
  • Ayano Shibata
  • Miku Hirane
  • Mutsumi Araki
  • Yan Dong
  • Nobuyuki Fukushima
  • Toshifumi Tsujiuchi


Lysophosphatidic acid (LPA) receptors (LPA1 to LPA6) indicate a variety of cellular responses, such as cell proliferation, migration, differentiation, and morphogenesis. However, the role of each LPA receptor is not functionally equivalent. Ethionine, an ethyl analog of methionine, is well known to be one of the potent liver carcinogens in rats. In this study, to assess whether ethionine may regulate cell motile activity through LPA receptors, rat liver epithelial (WB-F344) cells were treated with ethionine for 48 h. In cell motility assay with a cell culture insert, the treatment of ethionine at 1.0 and 10 μM enhanced significantly high cell motile activity, compared with untreated cells. The expression levels of LPA receptor genes in cells treated with ethionine were measured by quantitative real time RT-PCR analysis. The expression of the Lpar3 gene in ethionine-treated cells was significantly higher than that in untreated cells. Furthermore, to confirm an involvement of LPA3 on cell motility increased by ethionine, the Lpar3 knockdown cells were also used. The cell motile activity by ethionine was completely suppressed in the Lpar3 knockdown cells. These results suggest that LPA signaling through LPA3 may be involved in cell motile activity stimulated by ethionine in WB-F344 cells.


LPA LPA receptor-3 Cell motility Ethionine Liver 



Lysophosphatidic acid


LPA receptor-3


Reverse transcription


Polymerase chain reaction



This study was supported in part by a Grant-in-Aid (24590493) for Scientific Research (C) from Ministry of Education, Culture, Sports, Science and Technology, Japan, and by Grants from the Ministry of Health, Labor and Welfare of Japan.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Serina Inoue
    • 1
  • Eriko Tanabe
    • 1
  • Ayano Shibata
    • 1
  • Miku Hirane
    • 1
  • Mutsumi Araki
    • 1
  • Yan Dong
    • 1
  • Nobuyuki Fukushima
    • 2
  • Toshifumi Tsujiuchi
    • 1
  1. 1.Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and EngineeringKinki UniversityHigashiōsakaJapan
  2. 2.Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and EngineeringKinki UniversityHigashiōsakaJapan

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