Molecular and Cellular Biochemistry

, Volume 371, Issue 1–2, pp 137–146 | Cite as

Interleukin-22 protects rat PC12 pheochromocytoma cells from serum deprivation-induced cell death



Interleukin-22 (IL-22), an IL-10 family cytokine, mediates the crosstalk between leukocytes and epithelial cells. Previous studies reported that IL-22 expresses in mouse brain, and the rat PC12 cells are responsive to IL-22 stimulation. However, the biological roles of IL-22 in neuronal cells remain largely unknown. We show here that IL-22 activates Stat3, p38 mitogen-activated protein kinases (MAPK), and Akt pathways and inhibits Erk/MAPK pathway in naïve PC12 cells. We further demonstrate that IL-22 protects naïve PC12 cells from serum starvation-induced cell death via the Jak1/Stat3 and Akt pathways. We also show that IL-22 has no effects on naïve PC12 cell proliferation and cannot protect naïve PC12 cells from 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity. However, IL-22 exerts a dose-dependent protective effect on MPP+-induced neurodegeneration in nerve growth factor-differentiated PC12 cells. Overall, our data suggest that IL-22 might play a role in neurological processes. To our knowledge, this is the first report showing that IL-22 confers a neuroprotective function, which may provide a new therapeutic option for treatment of neurodegenerative diseases.


Interleukin-22 (IL-22) PC12 cell Neurodegeneration Cell survival 





Dulbecco’s modified Eagle’s medium


Fetal bovine serum


Growth-associated protein 43


Horse serum






Mitogen-activated protein kinases






3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide


Nerve growth factor





This work was supported by the National Science Foundation of China (30870792 and 81030025).


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Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Yongchun Liu
    • 1
    • 4
  • Wenyan Pan
    • 2
  • Shengmei Yang
    • 2
  • Xiaoying Wu
    • 2
  • Jianfu Wu
    • 2
  • Jun Ma
    • 3
  • Zengqiang Yuan
    • 3
  • Songshu Meng
    • 2
  1. 1.Northern Jiangsu People’s HospitalYangzhouChina
  2. 2.College of Bioscience and BiotechnologyYangzhou UniversityYangzhouChina
  3. 3.State Key Laboratory of Brain and Cognitive Sciences, Institute of BiophysicsChinese Academy of SciencesBeijingChina
  4. 4.Clinical Medical SchoolYangzhou UniversityYangzhouChina

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