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RNA interference against MDM2 suppresses tumor growth and metastasis in pancreatic carcinoma SW1990HM cells

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Abstract

In our previous study, the mouse double minute 2 (MDM2) was identified as one of the leading genes that promote the metastasis of pancreatic cancer (PC). However, the mechanism by which MDM2 promotes metastasis of PC is not understood. In this study, we show that down-regulation of MDM2 through lentivirus-mediated RNA interference could also suppress in vitro proliferation and in vivo tumor growth, and led to an obvious inhibition of both in vitro invasion and in vivo live metastases of SW1990HM cells which had an over-expression of MDM2 and a higher metastatic potential. Moreover, we also show that the down-regulation of MDM2 induced a significant decrease in MMP9, Ki-67 and increase in P53, E-Cadherin expression, and results in an altered expression of genes involved in metastasis, apoptosis, and cell proliferation. Our results suggest that MDM2 plays an important role in metastasis as well as tumor growth of PC. MDM2 could be a hopeful target for the control of PC.

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Acknowledgments

The authors thank Jianming Luo, Department of Breast Surgery, of the Breast Cancer Institute, Fudan University, for their help with this study. We also thank Lixin Chen for his help with the animal experiments.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Integrative Oncology, and Department of Oncology, Shanghai Medical College, 270 Dong An Road, Shanghai, 200032, People’s Republic of China

    Weidong Shi, Zhiqiang Meng, Zhen Chen, Yongqiang Hua, Huifeng Gao, Peng Wang, Junhua Lin, Zhenhua Zhou & Luming Liu

  2. Central Laboratory, Shanghai Cancer Center, Fudan University, 270 Dong An Road, Shanghai, 200032, People’s Republic of China

    Jianmin Luo

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  1. Weidong Shi
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  2. Zhiqiang Meng
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  3. Zhen Chen
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  4. Yongqiang Hua
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  5. Huifeng Gao
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  6. Peng Wang
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  7. Junhua Lin
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  8. Zhenhua Zhou
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  9. Jianmin Luo
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  10. Luming Liu
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Correspondence to Luming Liu.

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Shi, W., Meng, Z., Chen, Z. et al. RNA interference against MDM2 suppresses tumor growth and metastasis in pancreatic carcinoma SW1990HM cells. Mol Cell Biochem 387, 1–8 (2014). https://doi.org/10.1007/s11010-011-1208-4

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  • DOI: https://doi.org/10.1007/s11010-011-1208-4

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