In vitro study of the differentiation of bone marrow stromal cells into cardiomyocyte-like cells
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Bone marrow multipotent stromal cells (BMSCs) have the ability to transdifferentiate into various cell types, including: osteoblasts, chondrocytes, adipocytes, neurons, and cardiomyocytes. This study aimed to differentiate the BMSCs into cardiomyocyte. BMSCs were exposed to 5-azacytidine for 24 h. Seven days after the induction of cell differentiation by 5-azacytidine, the cardiomyogenic cells were stained by fushin and binucleated cells were counted and compared with the neonate cardiomyocyte as positive control. In addition, immunofluorescence analysis and western blot were performed using the antibodies against α-actinin, desmin, troponin T, and β-myosin heavy chain. Our results showed that there was no significant difference between the number of binucleated cells within the cardiomyogenic cell group and positive control group; however, a statistically significant difference was observed between both of these groups and undifferentiated cell group (P < 0.005). In addition, after 5-azacytidine treatment, BMSCs had a higher expression of cardiac-specific markers such as desmin, α-actinin, troponin T and β-myosin heavy chain compared with the untreated groups (P < 0.005). We concluded that 5-azacytidine is an effective inducer for the differentiation of BMSCs into cardiomyocytes and could produce a population of binucleated cells, which express α-actinin, desmin, troponin T, and β-myosin heavy chain, four markers of cardiomyocytes.
KeywordsBone marrow Cardiomyocyte-like cells 5-Azacytidine Desmin α-Actinin
We thank Dr. Moemeni for his constant encouragement and advice during the course of this work. We also thank Dr. Monavar for his kind helps and comments.
- 4.Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low WC, Largaespada DA, Verfaillie CM (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418:41–49PubMedCrossRefGoogle Scholar
- 7.Pittenger MF, Marshak DR (2001) Mesenchymal stem cells of human adult bone marrow. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- 17.Scorsin M, Marotte F, Sabri A, Le Dref O, Demirag M, Samuel JL, Rappaport L, Menasche P (1996) Can grafted cardiomyocytes colonize peri-infarct myocardial areas? Circulation 94:337–340Google Scholar