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Molecular and Cellular Biochemistry

, Volume 352, Issue 1–2, pp 109–115 | Cite as

Oxidized phosphatidylcholine induces migration of bone marrow-derived mesenchymal stem cells through Krüppel-like factor 4-dependent mechanism

  • Sang Hun Shin
  • Hae Young Song
  • Min Young Kim
  • Eun Kyung Do
  • Jung Sub Lee
  • Jae Ho Kim
Article

Abstract

Platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) and PAF-like oxidized phospholipids including 1-palmitoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine (POVPC) are generated upon LDL oxidation. The aim of this study was to evaluate the question of whether POVPC can regulate migration of human bone marrow-derived stem cells (hBMSCs) and to characterize signaling mechanisms involved in the POVPC-induced cell migration. POVPC treatment resulted in dose- and time-dependent increase of hBMSCs migration. Treatment of cells with BN52021, a specific antagonist of PAF receptor, completely blocked cell migration induced by not only PAF but also POVPC. Silencing of endogenous PAF receptor expression using PAF receptor-specific small interfering RNA resulted in significant attenuation of cell migration induced by PAF or POVPC. Both PAF and POVPC induced expression of Krüppel-like factor 4 (KLF4) in hBMSCs. POVPC- or PAF-induced cell migration was abrogated by small interfering RNA-mediated depletion of endogenous KLF4. These results suggest that PAF receptor plays a pivotal role in POVPC-induced migration of human BMSCs through PAF receptor-mediated expression of KLF4.

Keywords

POVPC Platelet-activating factor Krüppel-like factor 4 Mesenchymal stem cells Migration 

Abbreviations

LDL

Low density lipoprotein

PAF

Platelet-activating factor

PGPC

1-Palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine

POVPC

1-Palmitoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine

PAF-R

PAF receptor

VSMCs

Vascular smooth muscle cells

MSCs

Mesenchymal stem cells

hBMSCs

Human bone marrow-derived MSCs

RT-PCR

Reverse transcription-polymerase chain reaction

siRNA

Small interfering RNA

PDGF-BB

Platelet-derived growth factor-BB

Notes

Acknowledgments

This research was supported by a program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0020274) and by the MRC program (2010-0001251).

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Sang Hun Shin
    • 1
    • 2
  • Hae Young Song
    • 1
    • 2
  • Min Young Kim
    • 1
    • 2
  • Eun Kyung Do
    • 1
    • 2
  • Jung Sub Lee
    • 3
  • Jae Ho Kim
    • 1
    • 2
  1. 1.Medical Research Center for Ischemic Tissue RegenerationPusan National UniversityYangsanRepublic of Korea
  2. 2.Department of Physiology, School of MedicinePusan National UniversityYangsanRepublic of Korea
  3. 3.Department of Orthopaedic Surgery, School of MedicinePusan National UniversityBusanRepublic of Korea

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