Molecular and Cellular Biochemistry

, Volume 351, Issue 1–2, pp 233–241 | Cite as

A proteomic investigation into adriamycin chemo-resistance of human leukemia K562 cells

  • Xingchen Peng
  • Fengming Gong
  • Gang Xie
  • Yuwei Zhao
  • Minghai Tang
  • Luoting Yu
  • Aiping Tong


This study aimed to explore the mechanism of adriamycin resistance in human chronic myelogenous leukemia cells. Proteomic approach was utilized to compare and identify differentially expressed proteins between human chronic myelogenous leukemia K562 cells and their adriamycin-resistant counterparts. The differentially expressed proteins were analyzed by 2-DE (two-dimensional gel electrophoresis), and protein identification were performed on ESI-Q-TOF MS/MS instrument. Out of the 35 differentially expressed proteins between the two cell lines, 29 were identified and grouped into 10 functional classes. Most of identified proteins were related to the categories of metabolism (24%), proteolysis (13%), signal transduction (21%) and calcium ion binding (6%), suggesting that alterations of those biological processes might be involved in adriamycin resistance of K562 cells. We believe this study may provide some clues to a better understanding of the molecular mechanisms underlying adriamycin resistance.


Chronic myelogenous leukemia Drug resistance Adriamycin 2-DE Proteomics 



Two-dimensional gel electrophoresis




Coomassie brilliant blue


Dulbecco-modified Eagle medium


Isoelectric focusing


Immobilized pH gradient


Mass spectrometry


Phosphate-buffered saline


Polyvinylidene fluoride


Quadrupole time-of-flight


Tris-buffered saline Tween-20



This study was supported by the grants from the National Natural Sciences Foundation of China (30801294), Fok Ying Tung Education Foundation (201080) and the special major science and technology project for “creation of major new drugs” of China (2009ZX09103-132).

Conflict of interest

The authors have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Xingchen Peng
    • 1
    • 2
  • Fengming Gong
    • 1
  • Gang Xie
    • 1
  • Yuwei Zhao
    • 1
  • Minghai Tang
    • 1
  • Luoting Yu
    • 1
  • Aiping Tong
    • 1
  1. 1.State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical SchoolSichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of OncologyGuizhou Provincial People’s HospitalGuiyangChina

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