Myocardial fibrosis and TGFB expression in hyperhomocysteinemic rats
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Hyperhomocysteinemia, characterized by an elevated plasma homocysteine concentration, leads to several clinical manifestations and particularly cardiovascular diseases. Experimental models of hyperhomocysteinemia revealed several tissue injuries including heart fibrosis and ventricular hypertrophy. In order to analyze the molecular mechanisms link to these morphological alterations, a mild hyperhomocysteinemia was induced in rats via a chronic methionine administration. Effects of methionine administration were examined by histological analysis with Sirius red staining, histomorphometric analysis, zymography, and immunoblotting. Hyperhomocysteinemia due to methionine administration produces an interstitial myocardial fibrosis and a ventricular cardiomyocyte hypertrophy, which were associated with increased expression of transforming growth factor-beta1 (TGFβ1), tissue inhibitors of metalloproteinase (TIMP) 2, and JNK activation. However, the matrix metalloproteinase 2 activity was decreased in the hearts of hyperhomocysteinemic rats. Moreover, the TIMP1 protein expression was decreased, and the TIMP1–MMP1 balance was shifted. Remodeling in cardiac tissue observed in rat model of mild hyperhomocysteinemia is associated with a dysregulation in extracellular matrix degradation which results, at least in part, from enhancement of TGFβ1 level.
KeywordsHomocysteine Heart Ventricular cardiomyocyte hypertrophy Fibrosis MMP/TIMP TGFβ1
This study was supported in part by an EU grant AnEUploïdie. Lamia Raaf is supported by the Ministère de l’Enseignement Supérieur et de la Recherche Scientifique. Christophe Noll is supported by a fellowship from the Ministère de l’Enseignement supérieur et de la Recherche. We acknowledge the support from the technical platform “Quantitative microscopy with unbiased stereology” (Unité de Biologie Fonctionnelle et Adaptative, Université Paris Diderot-Paris 7, CNRS EAC 4413).
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