Amino acids influence the glucose uptake through GLUT4 in CHO-K1 cells under high glucose conditions
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According to studies earlier, amino acids have proven to be antidiabetic, antiglycating, and anticataractogenic. The present study was to explore whether amino acids as mixtures could enhance glucose uptake in CHO-K1 cells specifically. The cells in F-12K1 serum-free medium were exposed to normal (7 mM) and high glucose (12, 17 and 27 mM) in the presence and absence of amino acids mixture (AAM) in varying concentration (2.5, 5 and 10 mM). The mixture 5 and 10 mM AAM increased the 2-deoxyglucose (2DG) uptake at all glucose concentration significantly. There was also a significant increase in the GLUT4 (glucose transporter) translocation as revealed by flow cytometer. Addition of a mixture of amino acids was found to improve cell viability, which got altered by high glucose in the CHO-K1 cells. Amino acids as mixture had a beneficial effect in improving the net utilization of glucose as an additive effect with insulin.
KeywordsAmino acids Glucose uptake GLUT4
This work was supported by a grant from the Department of Science and Technology (DST) (Grant SR/SO/BB-15/2005), Govt. of India, New Delhi. We thank Dr. S. Ramakrishnan, Prof. Emeritus & Dr. K. N. Sulochana, Director, Department of Biochemistry and Cell Biology, Vision Research Foundation, Chennai, for their scientific support. The authors thank Dr. N. Rajagopal for his excellent technical assistance.
Conflicts of interest statement
All the authors state that they have no competing financial interests existing with respect to publication of this work.
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