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Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitochondria

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Abstract

Dopamine is a neurotransmitter that has been related to mitochondrial dysfunction. In this study, striatal intact mitochondria and submitochondrial membranes were incubated with different dopamine concentrations, and changes on mitochondrial function, hydrogen peroxide, and nitric oxide production were evaluated. A 35% decrease in state 3 oxygen uptake (active respiration state) was found after 1 mM dopamine incubation. In addition, mitochondrial respiratory control significantly decreased, indicating mitochondrial dysfunction. High dopamine concentrations induced mitochondrial depolarization. Also, evaluation of hydrogen peroxide production by intact striatal mitochondria showed a significant increase after 0.5 and 1 mM dopamine incubation. Incubation with 0.5 and 1 mM dopamine increased nitric oxide production in submitochondrial membranes by 28 and 49%, respectively, as compared with control values. This study provides evidence that high dopamine concentrations induce striatal mitochondrial dysfunction through a decrease in mitochondrial respiratory control and loss of membrane potential, probably mediated by free radical production.

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Acknowledgments

This research was supported by grants from Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas and Agencia Nacional de Promoción Científica y Tecnológica, Argentina.

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Authors and Affiliations

  1. Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina

    Analía Czerniczyniec, Juanita Bustamante & Silvia Lores-Arnaiz

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  1. Analía Czerniczyniec
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  2. Juanita Bustamante
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  3. Silvia Lores-Arnaiz
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Correspondence to Silvia Lores-Arnaiz.

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Czerniczyniec, A., Bustamante, J. & Lores-Arnaiz, S. Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitochondria. Mol Cell Biochem 341, 251–257 (2010). https://doi.org/10.1007/s11010-010-0456-z

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  • DOI: https://doi.org/10.1007/s11010-010-0456-z

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