Molecular and Cellular Biochemistry

, Volume 338, Issue 1–2, pp 175–181 | Cite as

Up-regulated α-actin expression is associated with cell adhesion ability in 3-D cultured myocytes subjected to mechanical stimulation

  • Yu Wang
  • Zhihe Zhao
  • Yu Li
  • Youwei Li
  • Jiapei Wu
  • Xiaofeng Fan
  • Pu Yang


This study was aimed to investigate the alteration of α-actin in three-dimensionally (3-D) cultured myocytes under cyclic tensile stress loading. Myocytes were collected from neonatal SD rat’s lateral pterygoid muscle for primary cell culture. The third-passage cells were implanted and 3-D cultured in poly lactic-co-glycolic acid (PLGA) scaffold, and then subjected to cyclic tensile stress (0.5 Hz, 2,000 μstrain) for 0, 2, 4, 8, 12, and 24 h through a four-point bending strain system. The α-actin mRNA was investigated by semi-quantitative RT–PCR. The α-actin protein expression was examined by immunofluorescent cytochemistry, laser confocal scanning microscopy (LCSM), and image analysis technology. The dynamic adhesion of myocytes to PLGA scaffolds was investigated by fluorescence microscope and the viability of the myocytes was measured by MTT assay. After mechanical loading, the α-actin mRNA increased at 2 h and then declined. The α-actin protein expression kept increased until peaked at 12 h, but declined at 24 h. The time course changing of α-actin protein expression parallelled with that of cell adhesion ability. It is concluded that α-actin expression is probably associated with cell adhesion ability in myocytes subjected to mechanical stimulation.


Tensile stress Myocyte α-actin 3-D culture Adhesion 



The research was supported by the grants from National Nature Science Foundation of China No. 10472138 and No. 30800212.


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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Yu Wang
    • 1
  • Zhihe Zhao
    • 1
  • Yu Li
    • 1
  • Youwei Li
    • 2
  • Jiapei Wu
    • 1
  • Xiaofeng Fan
    • 3
  • Pu Yang
    • 1
  1. 1.Department of Orthodontics, State Key Laboratory of Oral DiseasesWest China Stomatology Hospital Sichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of ProsthodonticsCollege of Stomatology, Chongqing Medical UnivesityChongqingPeople’s Republic of China
  3. 3.Department of OrthodonticsHainan Stomatology HospitalHaikouPeople’s Republic of China

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