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PCR-SSCP and sequence analysis of three Odontotermes spp. (Order: Isoptera; Family: Termitidae) on the basis of partial 16SrRNA gene

  • Mamtesh Kumari
  • Vijay Lakshmi Sharma
  • Monika Sodhi
  • Manishi Mukesh
  • Yogesh Shouche
  • Ranbir Chander Sobti
Article

Abstract

Partial 16S gene fragments were amplified by using specific primers in few species/populations of termites of the genus Odontotermes (Isoptera:Termitidae:Macrotermitinae), and the PCR products were subjected to SSCP analysis. Three haplotypes obtained were subjected to sequencing. The sequences obtained were characterized to see the frequencies of each nucleotide bases. High A + T content was observed. The inter-specific pairwise sequence divergence in Odontotermes spp. ranged from 0% to 4.8% across the entire 16S gene fragment. Identical sequences were found between two populations of O. horni. Individuals of different species having Type-I conformational pattern, i.e. O. obesus (-AI) and O. horni (-MI), as well as Type-II of O. obesus (-UII) and O. bhagwatii (-CHII) had no percent diversity. Phylogenetic trees drawn on the basis of distance Neighbour-joining method revealed clustering of individuals according to their genera and families.

Keywords

Divergence Haplotypes Isoptera 16S gene Neighbour-joining method Odontotermes Partial sequences PCR-SSCP Sequencing 

Notes

Acknowledgements

We acknowledge The Council of Scientific and Industrial research (CSIR), New Delhi, for financial support and Zoological Survey of India (ZSI), Kolkata, for species identification.

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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Mamtesh Kumari
    • 1
    • 2
    • 3
    • 4
  • Vijay Lakshmi Sharma
    • 1
    • 2
    • 3
    • 4
  • Monika Sodhi
    • 1
    • 2
    • 3
    • 4
  • Manishi Mukesh
    • 1
    • 2
    • 3
    • 4
  • Yogesh Shouche
    • 1
    • 2
    • 3
    • 4
  • Ranbir Chander Sobti
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of ZoologyPunjab UniversityChandigarhIndia
  2. 2.Department of BiotechnologyPunjab UniversityChandigarhIndia
  3. 3.National Bureau of Animal Genomic ResourcesKarnalIndia
  4. 4.National Centre of Cell SciencesPuneIndia

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