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Molecular and Cellular Biochemistry

, Volume 320, Issue 1–2, pp 93–100 | Cite as

Glycosylphosphatidylinositol-anchored arginine-specific ADP-ribosyltransferase7.1 (Art7.1) on chicken B cells: the possible role of Art7 in B cell receptor signalling and proliferation

  • Masaharu Terashima
  • Mai Takahashi
  • Makoto Shimoyama
  • Yoshinori Tanigawa
  • Takeshi Urano
  • Mikako Tsuchiya
Article

Abstract

Arginine-specific ADP-ribosyltransferase (Art) catalyzes the mono-ADP-ribosylation, in which it transfers a single ADP-ribose moiety of NAD to the arginine residue(s) of target proteins, and may regulate the function of the proteins or peptides in cellular processes. In vertebrates, Art family is consisted of seven members (Arts1–7), and these Arts are distributed among various tissues except B lymphocytes. Previously, we described molecular cloning, characterization and distribution of glycosylphosphatidylinositol (GPI)-anchored Arts, Art7.1 and Art7.2 (formerly, we referred as cgArt1 and cgArt2, respectively) in chicken tissues (Terashima et al (2005) Biochem J 389:853–861). Here, we demonstrate for the first time that Art7.1 was predominantly expressed on the surface of B cells from the bursa of Fabricius as a GPI-anchored form, as well as on T cells from the thymocytes. Furthermore, we show that the expression of Art7.1 molecules on B cells could modulate the B cell receptor (BCR) signalling and direct the B cell fate to maturation. Thus, our present observation sheds light on the Art molecule expressed on B cells and its possible functional role in BCR signalling.

Keywords

ADP-ribosyltransferase GPI-anchor B cells NAD DT40 cells 

Abbreviations

Art

ADP-ribosyltransferase

BCR

B cell receptor

ERK

Extracellular signal-regulated kinase

FITC

Fluorescein isothiocyanate

FACS

Fluorescence-activated cell sorter

GPI

Glycosylphosphatidylinositol

HBSS

Hanks’ balanced salt solution

PI-PLC

Phosphatidylinositol-specific phospholipase C

PMSF

Phenylmethylsulfonyl fluoride

WST-1

Water-soluble tetrazolium salt-1

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Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Masaharu Terashima
    • 1
  • Mai Takahashi
    • 1
  • Makoto Shimoyama
    • 1
  • Yoshinori Tanigawa
    • 1
  • Takeshi Urano
    • 1
  • Mikako Tsuchiya
    • 1
  1. 1.Department of BiochemistryShimane University Faculty of MedicineIzumoJapan

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