Molecular and Cellular Biochemistry

, Volume 318, Issue 1–2, pp 183–190 | Cite as

Hormone induced changes in lactase glycosylation in developing rat intestine

  • Kamaljit Kaur Chaudhry
  • Safrun Mahmood
  • Akhtar Mahmood


Lactase exists in both soluble and membrane-bound forms in suckling rat intestine. The distribution of lactase and its glycosylated isoforms in response to thyroxine or cortisone administration has been studied in suckling rats. 75% of lactase activity was detected, associated with brush borders, compared to 24% in the soluble fraction of 8-day-old rats. Thyroxine treatment enhanced soluble lactase activity to 34%, whereas particulate fraction was reduced to 67% compared to controls. Cortisone administration reduced soluble lactase activity from 24% in controls to 12% with a concomitant increase in membrane-bound activity to 89%. Western blot analysis revealed lactase signal, corresponding to 220 kDa in both the soluble and membrane fractions, which corroborated the enzyme activity data. The elution pattern of papain solubilized lactase from agarose—Wheat Germ agglutinin, or Concanavalin A or Jacalin agglutinin columns was different in the suckling and adult rat intestines. Also the elution profile of lactase activity from agarose-lectin columns was modulated in cortisone, thyroxine, and insulin injected pups, which suggests differences in glycosylated isoforms of lactase under these conditions. These findings suggest the role of these hormones in inducing changes in lactase glycosylation during postnatal development of intestine, which may contribute to adult-type hypolactasia in rats.


Lactase Phlorizin Hydrolase (LPH) Rat intestinal development Lactase glycosylation 



Ms.Kamalji Kaur Chaudhry was supported by a Senior Research Fellowship, from Indian Council of Medical Research, New Delhi.


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Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Kamaljit Kaur Chaudhry
    • 1
  • Safrun Mahmood
    • 2
  • Akhtar Mahmood
    • 1
  1. 1.Department of BiochemistryPanjab UniversityChandigarhIndia
  2. 2.Department of Experimental Medicine and BiotechnologyPostgraduate Institute of Medical Education and ResearchChandigarhIndia

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