Abstract
Lactase exists in both soluble and membrane-bound forms in suckling rat intestine. The distribution of lactase and its glycosylated isoforms in response to thyroxine or cortisone administration has been studied in suckling rats. 75% of lactase activity was detected, associated with brush borders, compared to 24% in the soluble fraction of 8-day-old rats. Thyroxine treatment enhanced soluble lactase activity to 34%, whereas particulate fraction was reduced to 67% compared to controls. Cortisone administration reduced soluble lactase activity from 24% in controls to 12% with a concomitant increase in membrane-bound activity to 89%. Western blot analysis revealed lactase signal, corresponding to 220 kDa in both the soluble and membrane fractions, which corroborated the enzyme activity data. The elution pattern of papain solubilized lactase from agarose—Wheat Germ agglutinin, or Concanavalin A or Jacalin agglutinin columns was different in the suckling and adult rat intestines. Also the elution profile of lactase activity from agarose-lectin columns was modulated in cortisone, thyroxine, and insulin injected pups, which suggests differences in glycosylated isoforms of lactase under these conditions. These findings suggest the role of these hormones in inducing changes in lactase glycosylation during postnatal development of intestine, which may contribute to adult-type hypolactasia in rats.
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References
Henning SJ (1981) Postnatal development coordination of feeding, digestion and metabolism. Am J Physiol 241:G119–G214
Rings EH, van Beers EH, Drasinski SD (1994) Lactase-origin, gene expression, localization and function. Nutr Res 14:775–797
Kuokkanen M, Enattah NS, Oksanen A, Savilahti E, Orpana A, Jarvela I (2003) Transcriptional regulation of the lactase-phlorizin hydrolase gene by polymorphisms associated with adult-type hypolactasia. Gut 52:647–652
Torres-Pinedo R, Mahmood A (1984) Postnatal changes in biosynthesis of microvillus membrane glycans of rat small intestine: I. Evidence of a developmental shift from terminal sialylation to fucosylation. Biochem Biophys Res Commun 125:546–553
Mahmood A, Torres-Pinedo R (1985) Effect of hormone administration on the sialylation and fucosylation of intestinal microvillus membranes of suckling rats. Pediatr Res 19:899–902
Lenoir D, Ruggiero-Lopez D, Louisot P, Biol MC (1995) Developmental changes in intestinal glycosylation: nutrition-dependent multi-factor regulation of the fucosylation pathway at weaning time. Biochim Biophys Acta 1234:29–36
Timofeeva NM, Gordova LA, Egorova VV, Iezuitova NN, Nikitina AA (2002) Membrane and soluble isoforms of intestinal ferments of young rats, whose mothers were on low protein diet during pregnancy and lactation. Zh Evol Biokhim Fiziol 38:146–152
Grunberg J, Luginbuhl U, Sterchi EE (1992) Proteolytic processing of human intestinal lactase-phlorizin hydrolase precursor is not a prerequisite for correct sorting in Madin Darby canine kidney (MDCK) cells. FEBS Lett 314:224–228
Buller HA, Rings EH, Pajkrt D, Montgomery RK, Grand RJ (1990) Glycosylation of lactase-phlorizin hydrolase in rat small intestine during development. Gastroenterology 98:667–675
Yeh KY, Yeh M, Hoff PR (1991) Thyroxine and cortisone cooperate to modulate postnatal intestinal enzyme differentiation in the rat. Am J Physiol 260:371–378
Biol MC, Lenoir D, Hugueny I, Louisot P (1992) Hormonal regulation of glycosylation processes in rat small intestine responsiveness of fucosyl transferase activity to hydrocortisone during the suckling period, unresponsiveness after weaning. Biochim Biophys Acta 1133:206–212
Chu SH, Walker WA (1986) Developmental changes in the activities of Sialyl- and fucosyltransferases in rat small intestine. Biochim Biophys Acta 883:496–500
Kessler M, Acuto O, Storelli C, Murer M, Semenza G (1978) A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of d-glucose and choline transport systems. Biochim Biophys Acta 506:136–154
Dahlqvist A (1964) Method for assay of intestinal disaccharidases. Anal Biochem 7:8–25
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lammeli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Mahmood A, Mahmood S, Desehryver-KecsKimeti K, Alpers DH (1993) Characterization of proteins in rat and human intestinal surfactant-like particles. Arch Biochem Biophys 300:280–286
Birkenmeier E, Alpers DH (1974) Enzymatic properties of rat lactase-phlorizin hydrolase. Biochim Biophys Acta 350:100–112
Yeh KY, Yeh M, Montgomery RK, Grand RJ, Hoff PR (1991) Cortisone and thyroxine modulate intestinal lactase and sucrase mRNA levels and activities in the suckling rat. Biochem Biophys Res Commun 180:174–180
King TP, Begbie R, Slater D, McFadyen M, Thom A, Kelly D (1995) Sialylation of intestinal microvillar membranes in newborn, sucking and weaned pigs. Glycobiology 5:525–534
Dai D, Nanthakumar NN, Savidge TC, Newburg DS, Walker WA (2002) Region-specific ontogeny of alpha-2, 6-sialyltransferase during normal and cortisone-induced maturation in mouse intestine. Am J Physiol Gastrointest Liver Physiol 282:G480–G490
Kaur K, Mahmood S, Mahmood A (2006) Susceptibility of lactase to luminal proteases in developing rat intestine. Indian J Gastroenterol 25:179–181
Chaudhary KK, Mahmood S, Mahmood A (2008) Hormone induced expression of brush border lactase in suckling rat intestine. Mol Cell Biochem 312:11–16
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Ms.Kamalji Kaur Chaudhry was supported by a Senior Research Fellowship, from Indian Council of Medical Research, New Delhi.
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Chaudhry, K.K., Mahmood, S. & Mahmood, A. Hormone induced changes in lactase glycosylation in developing rat intestine. Mol Cell Biochem 318, 183–190 (2008). https://doi.org/10.1007/s11010-008-9868-4
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DOI: https://doi.org/10.1007/s11010-008-9868-4