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Molecular and Cellular Biochemistry

, Volume 311, Issue 1–2, pp 105–110 | Cite as

Oxidative stress in primary glomerular diseases: a comparative study

  • Suchita Markan
  • Harbir Singh Kohli
  • Kamal Sud
  • Monica Ahuja
  • Tarunveer S. Ahluwalia
  • Vinay Sakhuja
  • Madhu Khullar
Article

Abstract

Objective To evaluate the status of oxidative stress in patients with different primary glomerular diseases (PGD) which have differential predisposition to renal failure. Methods Seventy-three patients with PGD and 50 controls were enrolled in the study. They were sub-grouped into non-proliferative glomerulonephritis (NPGN) and proliferative glomerulonephritis (PGN). Levels of serum malondialdehyde (MDA), reactive nitrogen intermediates (RNI), plasma total homocysteine (tHcy), urine 8-isoprostane (8-IP), RBC thiols, glutathione-S-transferase (GST) and serum superoxide dismutase (SOD) were measured spectrophotometrically. Results PGD patients showed a significant increase in MDA, RNI, tHcy, 8-IP levels (P < 0.05) and decreased SOD, total thiols and protein bound thiol levels as compared to controls (< 0.05). Significantly higher levels of tHcy, MDA and 8-IP (< 0.05) and lower SOD enzyme activity (< 0.05) were observed in PGN group as compared to NPGN and control groups. These changes remained significant even after adjustment was made for creatinine. Conclusions Oxidative stress in PGN is significantly higher than NPGN, indicating higher oxidative stress in these patients, independent of degree of renal dysfunction.

Keywords

Non-proliferative glomerulonephritis Oxidative stress Proliferative glomerulonephritis Reactive oxygen species. 

References

  1. 1.
    Noel LH, Gubler MC (2003) Histological classification of chronic glomerular diseases. Rev Prat 53:2005–2012PubMedGoogle Scholar
  2. 2.
    Araujo M, Welch WJ (2006) Oxidative stress and nitric oxide in kidney function. Curr Opin Nephrol Hypertens 15:72–77PubMedCrossRefGoogle Scholar
  3. 3.
    Wojcicka G, Beltowski J (2001) Oxidative stress in glomerulonephritis. Postepy Hig Med Dosw 55:855–869PubMedGoogle Scholar
  4. 4.
    Kuo HT, Kuo MC, Chiu YW, Chang JM, Guh JY, Chen HC (2005) Increased glomerular and extracellular malondialdehyde levels in patients and rats with focal segmental glomerulosclerosis. Eur J Clin Invest 35:245–250PubMedCrossRefGoogle Scholar
  5. 5.
    Gaertner SA, Janssen U, Koch OT, Floege KMJ, Gwinner W (2002) Glomerular oxidative and antioxidative systems in experimental mesangioproliferative glomerulonephritis. J Am Soc Nephrol 13:2930–2937PubMedCrossRefGoogle Scholar
  6. 6.
    Binder CJ, Weiher H, Exner M, Kerjaschki D (1999) Glomerular overproduction of oxygen radicals in Mpv17 gene-inactivated mice causes podocyte foot process flattening and proteinuria. A model of steroid-resistant nephrosis sensitive to radical scavenger therapy. Am J Pathol 154:1067–1075PubMedGoogle Scholar
  7. 7.
    Shah SV, Baliga R, Rajapurkar M, Fonseca VA (2007) Oxidants in chronic kidney disease. J Am Soc Nephrol 18:16–28PubMedCrossRefGoogle Scholar
  8. 8.
    Vaziri ND (2004) Roles of oxidative stress and antioxidant therapy in chronic kidney disease and hypertension. Curr Opin Nephrol Hypertens 13:93–99PubMedCrossRefGoogle Scholar
  9. 9.
    Himmelfarb J, Stenvinkel P, Ikizler TA, Hakim RM (2002) The elephant in uremia: oxidant stress as a unifying concept of cardiovascular disease in uremia. Kidney Int 62:1524–1538PubMedCrossRefGoogle Scholar
  10. 10.
    Ikizler TA, Morrow JD, Roberts LJ, Evanson JA, Becker B, Hakim RM, Shyr Y, Himmelfarb J (2002) Plasma F2-isoprostane levels are elevated in chronic hemodialysis patients. Clin Nephrol 58:190–197PubMedGoogle Scholar
  11. 11.
    Himmelfarb J, Hakim RM (2003) Oxidative stress in uremia. Curr Opin Nephrol Hypertens 12:593–598PubMedCrossRefGoogle Scholar
  12. 12.
    Oberg BP, McMenamin E, Lucas FL, McMonagle E, Morrow J, Ikizler TA, Himmelfarb J (2004) Increased prevalence of oxidant stress, inflammation in patients with moderate to severe chronic kidney disease. Kidney Int 65:1009–1016PubMedCrossRefGoogle Scholar
  13. 13.
    Chen HC, Tomino Y, Yaguchi Y et al (1991) Detection of polymorphonuclear cells, superoxide dismutase and poly C9 in glomeruli of patients with IgA nephropathy. Nephron 59:338PubMedGoogle Scholar
  14. 14.
    Bulucu F, Vural A, Aydin A, Sayal A (2000) Oxidative stress status in adults with nephrotic syndrome. Clin Nephrol 53:169–173PubMedGoogle Scholar
  15. 15.
    Poelstra K, Hardonk MJ, Koudstaal J, Bakker WW (1990) Intraglomerular platelet aggregation and experimental glomerulonephritis. Kidney Int 37:1500–1508PubMedCrossRefGoogle Scholar
  16. 16.
    Lapenna D, Cuccurullo F (1993) TBA test and “free” MDA assay in evaluation of lipid peroxidation and oxidative stress in tissue systems. Am J Physiol 265:1030–1032Google Scholar
  17. 17.
    Fiddler KM (1977) Collaborative study of modified AOAC method of analysis of nitrite in meat and meat products. J AOAC 60:594–598 Google Scholar
  18. 18.
    Jocelyn PC (1987) Spectrophotometric assay of thiols. Methods Enzymol 143:44–67PubMedCrossRefGoogle Scholar
  19. 19.
    Sexton DJ, Dimmock JR, Mutus B (1993) A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4, 4-dimethyl-5-diethylamino-1-penten-3-one hydrobromide. Biochem Cell Biol 71:98–101PubMedCrossRefGoogle Scholar
  20. 20.
    Durak I, Yurtarslanl Z, Canbolat O, Akyol O (1993) A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta 214:103–104PubMedCrossRefGoogle Scholar
  21. 21.
    Turi S, Nemeth I, Torkos A, Saghy L, Varga I, Matkovics B, Nagy J (1997) Oxidative stress and antioxidant defense mechanism in glomerular diseases. Free Radic Biol Med 22:161–168PubMedCrossRefGoogle Scholar
  22. 22.
    Perna AF, Ingrosso D, Lombardi C, Acanfora F, Satta E, Cesare CM, Violetti E, Romano MM, De Santo NG (2003) Possible mechanisms of homocysteine toxicity. Kidney Int Suppl 84:S137–S140PubMedCrossRefGoogle Scholar
  23. 23.
    Wollesen F, Brattstrom L, Refsum H, Ueland PM, Berglund L, Berne C (1999) Plasma total homocysteine and cysteine in relation to glomerular filtration rate in diabetes mellitus. Kidney Int 55:1028–1035PubMedCrossRefGoogle Scholar
  24. 24.
    Sinha I, Ghosh S, Dey P, Jacob J, Banerjee D (2005) Reduction of urinary thiols in nephrotic syndrome-a possible effect of free iron. Clin Chim Acta 355:91–96PubMedCrossRefGoogle Scholar
  25. 25.
    Stepniewska J, Ciechanowski K (2005) Oxidative stress as a reason of treatment difficulties in chronic renal failure. Pol Merkuriusz Lek 19:697–700Google Scholar
  26. 26.
    Ichikawa I, Kiyama S, Yoshioka T (1994) Renal antioxidant enzymes: their regulation and function. Kidney Int 45:1–9PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Suchita Markan
    • 1
  • Harbir Singh Kohli
    • 2
  • Kamal Sud
    • 2
  • Monica Ahuja
    • 1
  • Tarunveer S. Ahluwalia
    • 2
  • Vinay Sakhuja
    • 2
  • Madhu Khullar
    • 1
  1. 1.Department of Experimental Medicine and BiotechnologyPost Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia
  2. 2.Department of NephrologyPost Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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