Molecular and Cellular Biochemistry

, Volume 311, Issue 1–2, pp 31–36 | Cite as

Oral administration of phytocomponent p-hydroxycinnamic acid prevents bone loss in ovariectomized rats

  • Masayoshi Yamaguchi
  • Ying Ling Lai
  • Satoshi Uchiyama
  • Taeko Nakagawa


The preventive effect of phytocomponent p-hydroxycinnamic acid (HCA) on ovariectomy (OVX)-induced bone loss was investigated. HCA (250 or 500 μg/100 g body weight) was orally administered once daily for 30 days to OVX rats. The analysis using a peripheral quantitative computed tomography (pQCT) showed that OVX caused bone loss in the femoral-metaphyseal tissues. This change was significantly restored after the administration of HCA (250 or 500 μg/100 g body weight) to OVX rats. Mineral content, mineral density, and polar strength strain index in the femoral-metaphyseal tissues were significantly decreased in OVX rats. These decreases were significantly restored after the administration of HCA (500 μg/100 g) to OVX rats. Moreover, OVX caused a significant decrease in calcium content or alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues. These decreases were significantly restored after the administration of HCA (250 or 500 μg/100 g) to OVX rats. Deoxyribonucleic acid (DNA) content in the diaphyseal or metaphyseal tissues was significantly increased in OVX rats. These increases were significantly restored after oral administration of HCA (500 μg/100 g). This study demonstrates that HCA has preventive effects on OVX-induced bone loss of rats in vivo.


p-hydroxycinnamic acid Ovariectomy Bone loss Osteoporosis Rat femur 


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Copyright information

© Springer Science+Business Media, LLC. 2007

Authors and Affiliations

  • Masayoshi Yamaguchi
    • 1
    • 2
  • Ying Ling Lai
    • 1
  • Satoshi Uchiyama
    • 1
  • Taeko Nakagawa
    • 1
  1. 1.Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional SciencesUniversity of ShizuokaSuruga-kuJapan
  2. 2.Institute of Bone Health and NutrientAoi-kuJapan

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