Molecular and Cellular Biochemistry

, Volume 310, Issue 1–2, pp 153–158 | Cite as

Epicatechin gallate increases glutamate uptake and S100B secretion in C6 cell lineage

  • Renata T. Abib
  • André Quincozes-Santos
  • Patricia Nardin
  • Susana T. Wofchuk
  • Marcos L. Perry
  • Carlos-Alberto Gonçalves
  • Carmem Gottfried


There is a current interest in dietary compounds, such as green tea polyphenols, that can favor protection against a variety of brain disorders, including Alzheimer’s disease, ischemia, and stroke. The objective of the present study was to investigate the effects of (_)-epicatechin-3-gallate (ECG), one of three three major green tea antioxidants, on C6 lineage cells. Here, we evaluated cell morphology and integrity and specific astrocyte activities; glutamate uptake and secretion of S100B in the presence of 0.1, 1 and 10 μM ECG. During 6 h of incubation, cell morphology was altered only at 10 μM ECG; however, after 24 h of treatment, cells become stellate in the presence of all concentrations of ECG. Loss of cell integrity was observed after 24 h with 10 μM ECG and represented only 6% of cells, in contrast with 2% observed at basal conditions. ECG (1–10 μM) induced a decrease (about 36%) in glutamate uptake after 1 h of incubation. After 6 h, an opposite effect occurred and ECG induced a sustained increase in glutamate uptake of about 70% from 0.1 μM. In addition, a significant increase in S100B was observed at 1 μM ECG (36%) and 10 μM ECG (69%) after 1 h, in contrast to 6 h of treatment, where all doses of ECG induced a significant increase (about 60%) in S100B secretion. These data demonstrate that ECG induces a significant improvement in glutamate uptake and S100B secretion in C6 cells, indicating that ECG could contribute to the neuroprotective role of astroglial cells.


ECG Catechin Astrocyte C6 Glutamate uptake S100B 



This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and FINEP/Rede IBN 01.06.0842–00. We would like to thank Ms. Alessandra Heizelmann for technical support with cell culture.


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Copyright information

© Springer Science+Business Media, LLC. 2007

Authors and Affiliations

  • Renata T. Abib
    • 1
  • André Quincozes-Santos
    • 1
  • Patricia Nardin
    • 1
  • Susana T. Wofchuk
    • 1
  • Marcos L. Perry
    • 1
  • Carlos-Alberto Gonçalves
    • 1
  • Carmem Gottfried
    • 1
  1. 1.Departamento de BioquímicaUniversidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da SaúdePorto AlegreBrazil

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