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Molecular and Cellular Biochemistry

, Volume 308, Issue 1–2, pp 247–252 | Cite as

YY1 repressing peroxisome proliferator-activated receptor delta promoter

  • Cheng-Qiang He
  • Nai-Zheng Ding
  • Wei Fan
Article

Abstract

Peroxisome proliferator-activated receptors delta (PPARδ) is a nuclear hormone receptor belonging to the steroid receptor superfamily and is molecular targets for drugs to treat hypertriglyceridemia and type 2 diabetes. Yin Yang 1 (YY1) is a transcription factor that can repress or activate transcription of the genes with which it interacts. In this report, we show that YY1 specifically interacts with the PPARδ promoter. Overexpression of YY1 in Hela and NIH 3T3 cells repressed the activity of the PPARδ promoter, while the PPARδ promoter activity was enhanced when YY1 was knocked down by siRNA YY1. We also show that YY1 in nuclear extracts was able to bind the PPARδ promoter directly. These results suggest that YY1 might be a negative regulator of PPARδ gene expression through its direct interaction with the PPARδ promoter.

Keywords

PPAR delta YY1 Promoter 

Abbreviations

YY1

Yin Yang 1

MNDA

myeloid nuclear differentiation antigen

PPARs

peroxisome proliferator-activated receptors

PPARδ

peroxisome proliferator-activated receptors delta

RXR

retinoid X receptor

kb

kilobases

PBS

phosphate buffered saline

PCR

polymerase chain reaction

NIH 3T3

mouse embryonic fibroblast cell line

SEM

the standard error of measurement

EMSA

electrophoretic mobility shift assays

TBE

tris-Borate-EDTA

COS7

african green monkey SV40-transf’d kidney fibroblast cell line

Notes

Acknowledgements

This work was supported by Chinese National Natural Science Foundation grants 30400039. This work was also funded by Chinese Postdoctoral Foundation Grants 2003033069 and 2004036001.

We thank Dr. Shi Y for their generosity in supplying various siRNA expression vector pU6 yy1 and GFP siRNA expression plasmid, and Dr Galvagn F for their generosity in supplying pCMV-YY1, as indicated in the text, and Lu W for useful suggestions with drafting the manuscript.

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Copyright information

© Springer Science+Business Media, LLC. 2007

Authors and Affiliations

  1. 1.School of Biological Science and TechnologyCentral South UniversityChangshaChina
  2. 2.College of Life SciencePeking UniversityBeijingChina
  3. 3.College of Life ScienceShandong Normal UniversityShandong ProvinceChina
  4. 4.Qilu HospitalShandong ProvinceChina

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