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YY1 repressing peroxisome proliferator-activated receptor delta promoter

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Abstract

Peroxisome proliferator-activated receptors delta (PPARδ) is a nuclear hormone receptor belonging to the steroid receptor superfamily and is molecular targets for drugs to treat hypertriglyceridemia and type 2 diabetes. Yin Yang 1 (YY1) is a transcription factor that can repress or activate transcription of the genes with which it interacts. In this report, we show that YY1 specifically interacts with the PPARδ promoter. Overexpression of YY1 in Hela and NIH 3T3 cells repressed the activity of the PPARδ promoter, while the PPARδ promoter activity was enhanced when YY1 was knocked down by siRNA YY1. We also show that YY1 in nuclear extracts was able to bind the PPARδ promoter directly. These results suggest that YY1 might be a negative regulator of PPARδ gene expression through its direct interaction with the PPARδ promoter.

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Abbreviations

YY1:

Yin Yang 1

MNDA:

myeloid nuclear differentiation antigen

PPARs:

peroxisome proliferator-activated receptors

PPARδ:

peroxisome proliferator-activated receptors delta

RXR:

retinoid X receptor

kb:

kilobases

PBS:

phosphate buffered saline

PCR:

polymerase chain reaction

NIH 3T3:

mouse embryonic fibroblast cell line

SEM:

the standard error of measurement

EMSA:

electrophoretic mobility shift assays

TBE:

tris-Borate-EDTA

COS7:

african green monkey SV40-transf’d kidney fibroblast cell line

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Acknowledgements

This work was supported by Chinese National Natural Science Foundation grants 30400039. This work was also funded by Chinese Postdoctoral Foundation Grants 2003033069 and 2004036001.

We thank Dr. Shi Y for their generosity in supplying various siRNA expression vector pU6 yy1 and GFP siRNA expression plasmid, and Dr Galvagn F for their generosity in supplying pCMV-YY1, as indicated in the text, and Lu W for useful suggestions with drafting the manuscript.

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Authors and Affiliations

  1. School of Biological Science and Technology, Central South University, Changsha, Hunan Province, 410013, China

    Nai-Zheng Ding

  2. College of Life Science, Peking University, Beijing, 100871, China

    Nai-Zheng Ding

  3. College of Life Science, Shandong Normal University, Shandong Province, 250014, China

    Cheng-Qiang He

  4. Qilu Hospital, Shandong Province, 250014, China

    Wei Fan

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  1. Cheng-Qiang He
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  2. Nai-Zheng Ding
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  3. Wei Fan
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Correspondence to Nai-Zheng Ding.

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He, CQ., Ding, NZ. & Fan, W. YY1 repressing peroxisome proliferator-activated receptor delta promoter. Mol Cell Biochem 308, 247–252 (2008). https://doi.org/10.1007/s11010-007-9632-1

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  • DOI: https://doi.org/10.1007/s11010-007-9632-1

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