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Molecular and Cellular Biochemistry

, Volume 305, Issue 1–2, pp 163–169 | Cite as

Effect of adenosine in extracellular matrix synthesis in human and rat mesangial cells

  • Carlos Martínez-Salgado
  • Begoña García-Cenador
  • Isabel Fuentes-Calvo
  • Juan F. Macías Núñez
  • José M. López-Novoa
Article

Abstract

Adenosine (ADO) is an intermediary metabolite of adenosine trisphosphate degradation and a vasoactive mediator. We showed previously that ADO induces contraction and proliferation in rat mesangial cells by a mechanism involving A1 and A2 receptors. The studies concerning the effect of ADO on extracellular matrix (ECM) accumulation in mesangial cells are scarce. The purpose of our study was to evaluate the effect of ADO and the effect of the selective stimulation of A1 and A2 ADO receptors on the expression of ECM components fibronectin and collagen type I, in human and rat renal mesangial cells. Cultured human and rat renal mesangial cells were subjected to selective stimulation of A1 and A2 ADO receptors for 24 and 48 h. Fibronectin and collagen type I expression was evaluated by Western blot; total collagen synthesis was measured by [3H]-proline incorporation into collagen proteins. ADO, A1 and A2 receptor stimulation induce increases in fibronectin expression in rat mesangial cells, and A1 receptor stimulation partially inhibits fibronectin expression in serum-stimulated rat mesangial cells, without any effect in human mesangial cells. A2 receptor stimulation reduces collagen type I expression in serum-stimulated mesangial cells. Neither ADO nor A1 or A2 receptor stimulation induce significant changes in total collagen synthesis. These data suggest that ADO is not a major regulator of ECM synthesis in rat and human mesangial cells.

Keywords

Adenosine Collagen Extracellular matrix Fibronectin Fibrosis Mesangial cells Kidney 

Notes

Acknowledgments

This study was supported by grants from Instituto de Salud Carlos III (Ministerio de Sanidad y Consumo, CP03/00094, PI041817, and C03/10), Comisión Interministerial de Ciencia y Tecnología (BFU-2004-00285/BFI), and Junta de Castilla y León (SA001C05 y SA32/04). Immortalized human mesangial cells were a kind gift of Dr. Bernard Banas (Klinik und Poliklinik für Innere Medizin II, University of Regensburg, Regensburg, Germany)

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Carlos Martínez-Salgado
    • 1
  • Begoña García-Cenador
    • 2
  • Isabel Fuentes-Calvo
    • 2
  • Juan F. Macías Núñez
    • 3
  • José M. López-Novoa
    • 2
  1. 1.Unidad de InvestigaciónHospital Universitario de SalamancaSalamancaSpain
  2. 2.Departamento de Fisiología y Farmacología, Instituto “Reina Sofía” de Investigación NefrológicaUniversidad de SalamancaSalamancaSpain
  3. 3.Servicio de Nefrología, Hospital Universitario de SalamancaSalamancaSpain

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