Rhythmic clock gene expression in heart, kidney and some brain nuclei involved in blood pressure control in hypertensive TGR(mREN-2)27 rats
- 214 Downloads
Hypertensive TGR(mREN-2)27 rats exerting inverted blood pressure (BP) profile were used to study clock gene expression in structures responsible for BP control. TGR and control Sprague Dawley male rats were synchronized to the light:dark cycle 12:12 with food and water ad libitum. Daily rhythm in per2, bmal1, clock and dbp expression in the suprachiasmatic nucleus (SCN), rostral ventrolateral medulla (RVLM), nucleus of the solitary tract (NTS), heart and kidney was determined in both groups. Sampling occurred in regular 4 h intervals when rats of both strains were 11-weeks-old. Blood pressure and relative heart weight were significantly elevated in TGR rats in comparison with control. Expression of bmal1 and clock was up regulated in SCN of TGR rats but daily rhythm in per2 and dbp expression was similar in both groups. Mesor of per2 expression in RVLM was significantly higher in TGR than in control rats. In NTS of TGR rats expression of per2 was phase delayed by 3.5 h in comparison with control and bmal1 did not exert rhythmic pattern. Our study provided the first evidence about modified function of central and peripheral circadian oscillators in TGR rats at the level of clock gene expression. Expression of clock genes exerted up regulation in SCN and RVLM and down regulation in NTS. Circadian oscillators in selected brain structures were influenced more than oscillators in the heart and kidney by additional renin gene. Interactions of RAS and circadian system probably contribute to the development of inverted BP profile in TGR rats.
Key wordsclock genes medulla SCN blood pressure hypertension
Unable to display preview. Download preview PDF.
This study was supported by Science and Technology Assistance Agency under the contract No. APVT-20–022704 and SP 51/0280800/02808021. Authors are grateful to Dr. L. Červenka and Prof. M. Bader for providing experimental animals.
- 19.Palkovits M, Brownstein MJ (1988) Maps and Guide to Microdissection of the Rat Brain. Elsevier Science Publishing Co., New York, pp 1–242Google Scholar
- 22.Klemfuss H, Clopton PL (1993) Seeking tau: a comparison of six methods. J Interdisciplinary Cycle Res 24: 1–16 Google Scholar
- 37.Naito Y, Tsujino T, Kawasaki D, Okumura T, Morimoto S, Masai M, Sakoda T, Fujioka Y, Ohyanagi M, Iwasaki T (2003) Circadian gene expression of clock genes and plasminogen activator inhibitor-1 in heart and aorta of spontaneously hypertensive and Wistar-Kyoto rats. J Hypertens 21: 1107–1115PubMedCrossRefGoogle Scholar
- 47.Campos LA, Iliescu R, Baltatu O, Bader M: Hypertonie 2003: 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga (2003), Bonn, pp. Doc03hochV16Google Scholar