Molecular and Cellular Biochemistry

, Volume 295, Issue 1–2, pp 237–240 | Cite as

Deciphering the finger Prints of Brain Cancer Astrocytoma in comparison to Astrocytes by using near infrared Raman Spectroscopy

Short Communication


To explore the biochemical differences between brain cancer cells Astrocytoma and normal cells Astrocyte, we investigated the Raman spectra of single cell from these two cell types and analyzed the difference in spectra and intensity. Raman spectrum shows the banding pattern of different compounds as detected by the laser. Raman intensity measures the intensity of these individual bands.

The Raman spectra of brain cancer cells was similar to those of normal cells, but the Raman intensity of cancer cells was much higher than that of normal cells.

The Raman spectra of brain cancer Astrocytoma shows that the structural changes of cancer cells happen so that many biological functions of these cells are lost. The results indicate that Raman spectra can offer the experimental basis for the cancer diagnosis and treatment.


Raman spectroscopy Astrocytoma cancer optical tweezers 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.



This research is supported by the NSF-EPSCOR subcontract award# DTD42501, NASA grant# NAG512554 to H.B. and institutional NIH-EARDA grant# G11HD 34280-05. The authors are grateful to Dr. C. Xie and Dr. Y. Li of East Carolina University for their advise and help and Ms. Tiffany Jordan for help in preparation of the manuscript.


  1. 1.
    Banerjee H, Hawkins Z, Williams J, Blackshear M, Sawyer C, Cezares L, Pramanik SK, Williams A (2004) Search for a novel biomarker for the brain cancer Astrocytoma by using surface enhanced laser desorption/ionisation(SELDI) technique Cell Mol Biol 50: 733–736PubMedGoogle Scholar
  2. 2.
    Mannie MD, Norris MS (2001) MHC class-II-restricted antigen presentation by myelin basic protein-specific CD4-T cells causes prolonged desensitization and outgrowth of CD4-responders Cell Immunol 212: 51–62PubMedCrossRefGoogle Scholar
  3. 3.
    Mannie MD, Fraser DJ, McConnell TJ (2003) IL-4 responsive CD4-T cells specific for myelin basic protein: IL-2 confers a prolonged post activation refractory phase Immunol Cell Biol 81:8–19PubMedCrossRefGoogle Scholar
  4. 4.
    Norris MS, McConnell TJ, Mannie MD (2001) Interleukin-2 promotes antigenic reactivity of rested T cells but prolongs the post activation refractory phase of activated T cells Cell Immunol 211: 51–60PubMedCrossRefGoogle Scholar
  5. 5.
    Notingher I, Verrier S, Haque S, Polak JM, Hench LL (2003) Spectroscopic study of human lung epithelial cells (A579) in culture: living cells verses dead cells Biopolymers 72: 230–240PubMedCrossRefGoogle Scholar
  6. 6.
    Otto C, Sijtsema NM, Greve J (1998) Confocal Raman microspectroscopy of the activation of single neutrophilic granulocytes Eur Biophys J 27:582–590PubMedCrossRefGoogle Scholar
  7. 7.
    Patel DM, Dudek RW, Mannie MD (2001) Intercellular exchange of class II MHC complexes: ultrastructural localization and functional presentation of absorbed I-A/peptide complexes Cell Immunol 214:165–172PubMedCrossRefGoogle Scholar
  8. 8.
    Peticlolas WL, Patapoff TW, Thomas GA, Postlewait J, Powell JW (1996) Laser Raman microscopy of chromosomes in living eukaryotic cells: DNA polymorphism in vivo J Raman Spectosc 27: 571–580CrossRefGoogle Scholar
  9. 9.
    Puppels GJ, de Mul FF, Otto C, Greve J, Rovert-Nicoud M, Arndit-Jovin DJ, Jovin DJ, Jovin TM (1990) Studying single living cells and chromosomes by confocal Raman microspectroscopy Nature 347:301–303PubMedCrossRefGoogle Scholar
  10. 10.
    Puppels GJ, Garritsen HS, Segers-Nolten GM, de Mul FF, Greve J (1991) Raman microspectroscopic approach to the study of human granulocytes Biophys J 60:1046–1056PubMedGoogle Scholar
  11. 11.
    Salmaso BL, Puppels GJ, Caspers PJ, Ploris R, Wever R, Greve J (1994) Resonance Raman microspectroscopic characterization of eosinophil peroxidase in human eosinophilic granulocytes Biophys J 67:436–446PubMedGoogle Scholar
  12. 12.
    Sandstrom PA, Mannie MD, Buttke TM (1994) Inhibition of activation-induced death in T cell hybridomas by thiol antioxidants: oxidative stress as a mediator of apoptosis J Leukoc Biol 55:221–226PubMedGoogle Scholar
  13. 13.
    Sijtsema NM, Otto C, Segers-Nolten GM, Verhoeven AJ, Greve J (1998) Resonance Raman microspectroscopy of myeloperoxidase and cytochrome b558 in human neutrophilic granulocytes Biophys J 74:3250–3255PubMedCrossRefGoogle Scholar
  14. 14.
    Sijtsema NM, Tibb AG, Segers-Nolten IG, Verhoeven AJ, Weening RS, Greve J, Otto C (2000) Intracellular reactions in single human granulocytes upon phorbol myristate acetate activation using confocal Raman microspectroscopy Biophys 78:2606–2613Google Scholar
  15. 15.
    Xie CA, Li YQ (2003) Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques J Appl Phys 93: 2982–2986CrossRefGoogle Scholar
  16. 16.
    Xie CA, Dinno MA, Li YQ (2002) Near-infrared: Raman spectroscopy of single optically trapped biological cells Opt Lett 27: 249–251PubMedGoogle Scholar
  17. 17.
    Xie CA, Li YQ, Tang W, Newton RJ (2002) Study of dynamical process of heat denaturation in optically trapped single microorganisms by near-infrared Raman spectroscopy J Appl Phys 94: 6138–6142CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Biological SciencesElizabeth City State University, University of North CarolinaElizabeth CityUSA
  2. 2.Department of Physics and ChemistryElizabeth City State University, University of North CarolinaElizabeth CityUSA

Personalised recommendations