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Molecular and Cellular Biochemistry

, Volume 293, Issue 1–2, pp 193–201 | Cite as

Treatment with dehydroepiandrosterone (DHEA) stimulates oxidative energy metabolism in the liver mitochondria from developing rats

  • Minal A. Patel
  • Surendra S. Katyare
Article

Abstract

Effects of treatment with DHEA (0.2 mg or 1.0 mg / kg body weight for 7 days) on oxidative energy metabolism on liver mitochondria from developing and young adult rats were examined. Treatment with DHEA resulted in a progressive dose-dependent increase in the liver weights of the developing animals without change in the body weight. In the young adult rats treatment with 1.0 mg DHEA showed increase only in the body weight. Treatment with DHEA stimulated state 3 and state 4~respiration rates in developing as well as young adult rats in dose-dependent manner with all the substrates used; magnitude of stimulation was age-dependent. In young adults the extent of simulation of state 3 respiration rates declined at higher dose (1.0~mg) of DHEA with glutamate and succinate as substrates. Stimulation of state 3 respiration rates was accompanied by increase in contents of cytochrome aa3, b and c + c1 and stimulation of ATPase and dehydrogenases activities in dose- and age-dependent manner.

Keywords

Dehydroepiandrosterone (DHEA) oxidative energy metabolism cytochrome ATPase activity dehydrogenases liver mitochondria 

Abbreviations:

ADP

adenosine diphosphate

DCIP

dicholrophenolindophenol

DHEA

dehydroepiandrosterone

EDTAd

disodium salt of ethylenediaminetetraacetic aci

MOPS

4-morpholinopropanesulfonic acid

TMPD

N,N,N',N'-tetra methyl-pphenylenediamine

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Minal A. Patel
    • 1
  • Surendra S. Katyare
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceThe Maharaja Sayajirao University of Baroda, VadodaraGujaratIndia

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