Molecular and Cellular Biochemistry

, Volume 293, Issue 1–2, pp 53–62 | Cite as

Relative suppression of the sodium-dependent Vitamin C transport in mouse versus human lens epithelial cells

  • Mark E. Obrenovich
  • Xingjun Fan
  • Makoto Satake
  • Simon M. Jarvis
  • Lixing Reneker
  • John R. Reddan
  • Vincent M. Monnier


Vitamin C is a major antioxidant and UV absorbent in the human lens. In the rodent lens, the levels are very low for unknown reasons. Searching for clues to explain this suppression, we investigated the comparative uptake of Vitamin C in cultured human and mouse lens epithelial cells. When compared to human HLE-B3 lens epithelial cells, 14C-ASA uptake was 4- to 10-fold impaired in confluent mouse lens 17EM15 (p < 0.0001) and 21EM15 (p < 0.001) cells, respectively. High glucose concentrations reduced the uptake by 30–50% in all cells (p < 0.005). Incubation of cells with 6-deoxy-6-fluoro-ascorbic (F-ASA), i.e. a probe specific for the sodium-dependent Vitamin C uptake (SVCT2), revealed a 10-fold uptake suppression into mouse 17EM15 relative to human HLE-B3 and JAR choriocarcinoma cells (a control), that could be overcome by overexpressing hSVCT2 using two different promoter constructs. The relative Vitamin C uptake differences suggest either low expression of SVCT2, molecular differences between the transporters themselves or their biological regulation, since a recent study has shown that exogenous feeding of ascorbic acid to rats increased only modestly lenticular uptake (Mody et al., Acta Ophthalmol Scand 83: 228–223, 2005). Elucidation of the mechanism by which SCVT2 activity is suppressed in mouse lens may help unravel a major question of evolutionary significance for night vision in nocturnal animals.

Key words

aging ascorbic acid diabetes glycation oxidation SVCT2 


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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Mark E. Obrenovich
    • 1
  • Xingjun Fan
    • 1
  • Makoto Satake
    • 1
    • 7
  • Simon M. Jarvis
    • 3
  • Lixing Reneker
    • 4
  • John R. Reddan
    • 5
  • Vincent M. Monnier
    • 1
    • 2
    • 6
  1. 1.Department of PathologyCase Western Reserve UniversityClevelandUSA
  2. 2.Department of BiochemistryCase Western Reserve UniversityClevelandUSA
  3. 3.Research School of BiosciencesUniversity of WestminsterLondonUK
  4. 4.Department of OphthalmologyUniversity of MissouriColumbiaUSA
  5. 5.Department of Biological SciencesOakland UniversityRochesterUSA
  6. 6.Institute of PathologyCase Western Reserve UniversityClevelandUSA
  7. 7.Tissue Engineering LaboratoryInnovation Research Institute, Teijin Ltd.TokyoJapan

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