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Molecular and Cellular Biochemistry

, Volume 286, Issue 1–2, pp 133–138 | Cite as

The effect of repeated tryptophan administration on body weight, food intake, brain lipid peroxidation and serotonin immunoreactivity in mice

  • Şule Coşkun
  • Çiğdem Özer
  • Bilge Gönül
  • Gülnur Take
  • Deniz Erdoğan
Article

Abstract

Tryptophan as a circulating precursor of serotonin (5-HT) may suppress food intake and body weight. Tryptophan administration can enhance the generation of reactive oxygen species (ROS) by inducing oxidative pathway in vivo and in vitro. We have examined the effect of repeated tryptophan administration on food consumption, body weight, brain lipid peroxidation and 5-HT immunoreactivity. Tryptophan was given at the dose of 100 mg/kg/24 hr in 0.2 ml saline solution i.p. for 7 days to mice. Control mice received 0.9% NaCL solution at the same manner and volume. Body weights were recorded at the beginning and end of the experiments. Thiobarbituric acid reactive substance (TBARS), the last product of lipid peroxidation, was measured spectrophotometrically. Brain 5-HT levels were determined by the immunohistochemical method. Our findings indicate that the tryptophan suppresses food intake significantly in mice. Body weight decreased and brain TBARS levels increased significantly by repeated tryptophan treatment. Immunohistochemical detection showed that 5-HT levels increased by tryptophan administration. There is a link between increased 5-HT level and oxidative stress by tryptophan administration on brain tissue. Tryptophan at repeated doses should be exercised carefully in clinical practice.

Key words

brain lipid peroxidation food intake glutathione serotonin immunoreactivity tryptophan 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Şule Coşkun
    • 1
    • 4
  • Çiğdem Özer
    • 2
  • Bilge Gönül
    • 2
  • Gülnur Take
    • 3
  • Deniz Erdoğan
    • 3
  1. 1.Department of Biology, Art and Science FacultyGazi UniversityAnkaraTurkey
  2. 2.Department of Physiology, Faculty of MedicineGazi UniversityAnkaraTurkey
  3. 3.Department of Histology, Faculty of MedicineGazi UniversityAnkaraTurkey
  4. 4.Science and Art Faculty, Department of Biology, Physiology, TeknikokullarGazi UniversityAnkaraTurkey

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