Molecular and Cellular Biochemistry

, Volume 278, Issue 1–2, pp 93–100 | Cite as

Region-specific effects of hypothyroidism on the relative expression of thyroid hormone receptors in adult rat brain

  • Caterina Constantinou
  • Marigoula Margarity
  • Theony Valcana


The aim of this study was to determine whether changes in the circulating thyroid hormone (TH) and brain synaptosomal TH content affected the relative levels of mRNA encoding different thyroid hormone receptor (TR) isoforms in adult rat brain. Northern analysis of polyA+RNA from cerebral cortex, hippocampus and cerebellum of control and hypothyroid adult rats was performed in order to determine the relative expression of all TR isoforms. Circulating and synaptosomal TH concentrations were determined by radioimmunoassay. Region-specific quantitative differences in the expression pattern of all TR isoforms in euthyroid animals and hypothyroid animals were recorded. In hypothyroidism, the levels of TRα2 mRNA (non-T3-binding isoform) were decreased in all brain regions examined. In contrast the relative expression of TRα1 was increased in cerebral cortex and hippocampus, whereas in cerebellum remained unaffected. The TRβ1 relative expression in cerebral cortex and hippocampus of hypothyroid animals was not affected, whereas this TR isoform was not detectable in cerebellum. The TR isoform mRNA levels returned to control values following T4 intraperitoneal administration to the hypothyroid rats. The obtained results show that in vivo depletion of TH regulates TR gene expression in adult rat brain in a region-specific manner. (Mol Cell Biochem 278: 93–100, 2005)

Key Word

adult rat brain hypothyroidism methimazole thyroid hormone receptor isoforms (TR) 


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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Caterina Constantinou
    • 1
  • Marigoula Margarity
    • 1
  • Theony Valcana
    • 1
  1. 1.Laboratory of Human and Animal Physiology, Department of BiologyUniversity of PatrasPatrasGreece

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