Advertisement

Molecular and Cellular Biochemistry

, Volume 269, Issue 1, pp 131–136 | Cite as

Effect of Tridax procumbens on liver antioxidant defense system during lipopolysaccharide-induced hepatitis in D-galactosamine sensitised rats

  • Vilwanathan Ravikumar
  • Kanchi Subramanian Shivashangari
  • Thiruvengadam Devaki
Article

Abstract

The present study was carried out to assess the effect of chloroform insoluble fraction of ethanolic extract of Tridax procumbens (TP) against D-Galactosamine/Lipopolysaccharide (D-GalN/LPS)-induced hepatitis in rats. Induction of rats with D-GalN/LPS (300 mg/kg body weight/30 μg/kg body weight) led to a marked increase in lipid peroxidation as measured by thiobarbituric acid reactive substances (TBARS) in liver. Further there was a decline in the activities of enzymic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase and the levels of non-enzymic antioxidants namely reduced glutathione, vitamin C and vitamin E. These biochemical alterations were normalised upon pretreatment with TP extract. Thus, the above results suggest that TP (300 mg/kg body weight orally for 10 days) is very effective in allievating the D-GalN/LPS-induced oxidative stress suggesting its antioxidant property. (Mol Cell Biochem 269: 131–136, 2005)

Keywords

antioxidants D-Galactosamine hepatitis lipid peroxidation lipopolysaccharide Tridax procumbens 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cullen WJ, Dulchavsky SA, Devasagayam TPA, Venkatraman BV, Dutta S: Effect of Maharishi AK-4 on H2O2-induced oxidative stress in isolated rat hearts. J Ethnopharmacol 56: 215–222, 1997Google Scholar
  2. 2.
    Ames BN, Gold LS, Willet WC: The causes and prevention of cancer. Proc Natl Acad Sci 92: 5258–5265, 1995Google Scholar
  3. 3.
    Lin JM, Lin CC, Chen MF, Ujiie T, Takada A: Radical scavenger and antihepatotoxic activity of Ganoderma formosanum Ganoderma lucidum and Ganaderma neo-japonicum. J Ethnopharmacol 47: 33–41, 1995Google Scholar
  4. 4.
    Babu BH, Shylesh BS, Padikkala J: Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia 72: 272–277, 2001Google Scholar
  5. 5.
    Diwan PV, Karwande I, Margaret I, Sattur PB: Pharmacology and biochemical evaluation of Tridax procumbens on inflammation. Indian J Pharmacol 21: 1–7, 1989Google Scholar
  6. 6.
    Pathak AK, Saraf S, Dixit VK: Hepatoprotective activity of Tridax procumbens. Part I. Fitoterapia 62: 307–317, 1991Google Scholar
  7. 7.
    Saraf S, Pathak AK, Dixit VK: Hepatoprotective activity of Tridax procumbens Part II. Fitoterapia 62: 534–536, 1991Google Scholar
  8. 8.
    Taddei A, Rosas RAJ: Bioactivity studies of extracts from Tridax procumbens. Phytomed 7: 235–238, 2000Google Scholar
  9. 9.
    Udupa SL, Udupa AL, Kulkarni DR: Influence of Tridax procumbens on lysyl oxidase activity and would healing. Planta Medica 57: 325–327, 1991Google Scholar
  10. 10.
    Perumal SR, Ignacimuthu S, Raja DP: Preliminary screening of ethnomedicinal plants from India. J Ethnopharmacol 66: 235–240, 1999Google Scholar
  11. 11.
    Omar HM, Sanders RA, Watkins JB: Minimal effect of acute experimental hepatitis induced by lipopolysaccharide/D-galactosamine on biotransformation in rats. Biochem Pharmacol 52: 1921–1924, 1996Google Scholar
  12. 12.
    Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351–358, 1979PubMedGoogle Scholar
  13. 13.
    Misra HP, Fridovich I: The role of superoxide anion in the auto oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247: 3170–3175, 1972Google Scholar
  14. 14.
    Beers RF, Sizer IW: A spectroscopic method for measuring the breakdown of the hydrogen peroxide by catalase. J Biol Chem 195: 133–140, 1952Google Scholar
  15. 15.
    Rotruck JT, Pop AL, Ganther HF, Hafeman BG, Hoeksira WG: Selenium: Biochemical role as a component of glutathione peroxidase. Science 179: 588–590, 1973PubMedGoogle Scholar
  16. 16.
    Habig HW, Pabst MJ, Jakoby WB: Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249: 7130–7139, 1974Google Scholar
  17. 17.
    Moron MS, Depierre JW, Mannervik B: Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochem Biophys Acta 582: 62–68, 1979Google Scholar
  18. 18.
    Omaye ST, Turnbull TO, Sauberlich HE: Selected methods for the determination of ascorbic acid in cells, tissues and fluids. Methods Enzymol 62: 3–11, 1979Google Scholar
  19. 19.
    Desai ID: Vitamin E analysis methods for animal tissues. Methods Enzymol 105: 138–147, 1984Google Scholar
  20. 20.
    Markov AK, Farias LA, Bennett WS, Subramony C, Mibas AA: Prevention of galactosamine-induced hepatotoxicity in rats with fructose-1,6-diphosphate. Pharmacology 43: 310–317, 1991Google Scholar
  21. 21.
    Decker K: Mechanisms and mediators in hepatic necrosis. Gastroenterol Jpn 28: 20–25, 1993Google Scholar
  22. 22.
    Wendel A, Tiegs G: Leukotriene D4 mediates galactosamine/endotoxin-induced hepatitis in mice. Biochem Pharmacol 36: 1867–1870, 1987Google Scholar
  23. 23.
    Anukarahanota T, Shinozuka H, Farber E: Inhibition of protein synthesis in rat liver by D-galactosamine. Res Comm Chem Path Pharm 5: 482–491, 1973Google Scholar
  24. 24.
    Galanos CM, Freudenberg A, Reuter W: Galactosamine-induced sensitization to the lethal effect of endotoxin. Proc Natl Acad Sci 76: 5939–5943, 1979Google Scholar
  25. 25.
    Lin SC, Lin CC, Lu FJ, Lin YH, Chen CH: Protective and therapeutic effects of huanglian-jie-du-tang on hepatotoxin-induced liver injuries. Am J Chin Med 24: 219–229, 1996Google Scholar
  26. 26.
    Rodriguez S, Ancheta O, Ramos ME, Remirez D, Rojas E, Gonzalez R: Effects of cuban red propolis on galactosamine-induced hepatitis in rats. Pharmacol Res 35: 1–4, 1997Google Scholar
  27. 27.
    Shiratori Y, Kawase T, Shiina S, Okano K, Sugimoto T, Teraoka H, Matano S, Matsumoto K, Kamii K: Modulation of hepatoxicity by macrophages in the liver. Hepatol 8: 815–821, 1988Google Scholar
  28. 28.
    Hu HL, Chen RD: Changes in free radicals, trace elements by D-galactosamine. Biol Trace Elem Res 34: 19–25, 1992Google Scholar
  29. 29.
    Sun F, Hamagawa E, Tsutsui C, Sakaguchi N, Kakuta Y, Tokumaru S, Kojo S: Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. Biochem Pharmacol 65: 101–107, 2003Google Scholar
  30. 30.
    Neihorster M, Inoue M, Wendel A: A link between extracellular reactive oxygen and endotoxin-induced release of tumour necrosis α in vivo. Biochem Pharmacol 43: 1151–1154, 1992Google Scholar
  31. 31.
    Rajakrishnan V, Viswanathan P, Menon VP: Hepatotoxic effect of alcohol on female rats and siblings: Effects of N-acetylcysteine. Hepatol Res 9: 37–50, 1997Google Scholar
  32. 32.
    Sreepriya M, Devaki T: Effect of Indigofera tinctoria Linn. on liver antioxidant defense system during D-galactosamine/endotoxin-induced acute hepatitis in rodents. Indian J Exp Biol 39: 181–184, 2001Google Scholar
  33. 33.
    Comporti M: Lipid peroxidation and cellular damage in toxic liver injury. Lab Invest 53: 599–603, 1985PubMedGoogle Scholar
  34. 34.
    Sakaguchi S, Yokota K: Role of Ca2+ on endotoxin-sensitivity by galactosamine challenge: lipid peroxide formation and hepatotoxicity in zymosan-primed mice. Pharmacol Toxicol 77: 81–86, 1995Google Scholar
  35. 35.
    Vijayalakshmi T, Muthulakshmi V, Sachdanandam P: Salubrious effect of Semecarpus anacardium against lipid peroxidative changes in adjuvant arthritis studied in rats. Mol Cell Biochem 175: 65–69, 1997Google Scholar
  36. 36.
    Disilvestro RA, Rogers DL, Lee HG: Diethyldithio carbamate inhibition of galactosamine-induced hepatitis in rats. Pharmacol Res 45(3): 253–255, 2002Google Scholar
  37. 37.
    Limaye PV, Raghuram N, Sivakami S: Oxidative stress and gene expression of antioxidant enzymes in the renal cortex of streptozotocin-induced diabetic rats. Mol Cell Biochem 243: 147–152, 2003Google Scholar
  38. 38.
    Anandan R, Pandima Devi K, Devaki T, Govindaraju P: Preventive effects of Picrorhiza kurroa on D-galactosamine-induced hepatitis in rats. J Clin Biochem Nutr 25: 87–95, 1998Google Scholar
  39. 39.
    Fridovich I: Superoxide dismutase. Ann Res Biochem 44: 147–159, 1975Google Scholar
  40. 40.
    Vimal V, Devaki T: Hepatoprotective effect of allicin on tissue defense system in galactosamine/endotoxin challenged rats. J Ethnopharmacol 90: 151–154, 2004Google Scholar
  41. 41.
    Rister M, Banchner RL: The alteration of superoxide dismutase, catalase, glutathione peroxides, NAD(P)H, cytochrome reductase in guinea pig polymorphonuclear leucocytes and alveolar macrophage during hyperoxia. J Clin Invest 58: 1174–1184, 1976Google Scholar
  42. 42.
    Umalakshmi K, Devaki T: Effect of garlic oil on mitochondrial lipid peroxidation induced by ethanol. Med Sci Res 20: 435–437, 1992Google Scholar
  43. 43.
    Ishigaki S, Abramovitz M, Listowsky I: Glutathione-S-transferases are major cytosolic thyroid hormone binding proteins. Arch Biochem Biophys 273: 265–272, 1989Google Scholar
  44. 44.
    Ahmad A, Pillai KK, Najmi AK, Ahmad SJ, Pal SN, Balani DK: Evaluation of hepatoprotective potential of jigrine post-treatment against thioacetamide induced hepatic damage. J Ethnopharmacol 79: 35–41, 2002Google Scholar
  45. 45.
    Burk R: Glutathione-dependent protection by rat liver microsomal protein against lipid per oxidation. Biochem Biophys Acta 757: 21–28, 1983Google Scholar
  46. 46.
    Lim HK, Kim HS, Choi HS, OH S, Jang CG, Choi J, Kim SH, Chang MJ: Effects of acetylbergenin against D-GalN-induced hepatotoxicity in rats. Pharmacol Res 42: 471–474, 2000Google Scholar
  47. 47.
    Kyle ME, Nakal D, Saskaida I, Serroni A, Farber JL: Protein thiol depletion and the killing of cultured hepatocytes by hydrogen peroxide. Biochem Pharmacol 38: 3797–3805, 1989Google Scholar
  48. 48.
    Frie B, England L, Ames BN: Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA 86: 6377–6381, 1989Google Scholar
  49. 49.
    Dutta Roy AK, Gordon MJ, Campbell FM, Duthie GG, James WPT: Vitamin E requirements, transport and metabolism. Role of α-tocopherol binding proteins. J Nutr Biochem 5: 562–570, 1994Google Scholar
  50. 50.
    Komburst DJ, Mavis RD: Relative susceptibility of microsomes from lung, heart, liver, kidney, brain and testes to lipid peroxidation correlation with vitamin E content. Lipids 15: 315–322, 1980Google Scholar
  51. 51.
    Kadarian C, Broussalis AM, Mino J, Lopez P, Gorzalczany S, Ferraro G, Acevedo C: Hepatoprotective activity of Achyrocline satureioides (LAM) D.C. Pharmacol Res 45: 57–61, 2002Google Scholar
  52. 52.
    Klopman G, Dimayuga ML: Computer–automated structure evaluation of flavonoids and other structurally–related components as glyoxalase enzyme inhibitors. Mol Pharmacol 34: 218–222, 1988Google Scholar
  53. 53.
    Ali M, Ravinder E, Ramachandran R: A new flavonoid from the aerial parts of Tridax procumbens. Fitoterapia 72: 313–315, 2001Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Vilwanathan Ravikumar
    • 1
  • Kanchi Subramanian Shivashangari
    • 1
  • Thiruvengadam Devaki
    • 1
  1. 1.Department of BiochemistryUniversity of MadrasChennaiIndia

Personalised recommendations