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The ATPase subunit 6 gene of Leptomonas seymouri (Trypanosomatidae) is transcribed and edited as a polycistronic mRNA

  • Genomics. Transcriptomics. Proteomics
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Abstract

The maturation of mRNA was studied for the Leptomonas seymouri mitochondrial A6 gene coding for ATPase subunit 6. RT-PCR showed that the cytochrome b and A6 genes are transcribed to produce a polycistronic RNA. The RT-PCR products, containing the cDNAs of these genes, were cloned and sequenced. Comparison of the sequence for 12 clones revealed four types of transcripts representing partly or completely edited products of A6. Two different variants of completely edited A6 mRNAs were found.

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REFERENCES

  1. Stiles J., Micock P., Shah P., Meade J. 1999. Genomic organization, transcription, splicing and gene regulation in Leishmania. Ann. Trop. Med. Parasitol. 93, 781–807.

    Google Scholar 

  2. Clayton D.A. 2000. Vertebrate mitochondrial DNA: A circle of surprises. Exp. Cell Res. 255, 4–9.

    Google Scholar 

  3. Reichert A., Rothbauer U., Merl M. 1998. Processing and editing of overlapping tRNA in human mitochondria. J. Biol. Chem. 273, 31977–31984.

    Google Scholar 

  4. Brennicke A., Marchfelder A., Binder S. 1998. RNA editing. FEMS Microbiol. Rev. 23, 297–316.

    Google Scholar 

  5. Alfonzo J.D., Thiemann O., Simpson L. 1997. The mechanism of U insertion/deletion RNA editing in kine-toplastid mitochondria. Nucleic Acids Res. 25, 3751–3759.

    Google Scholar 

  6. Estevez A.M., Simpson L. 1999. Uridine insertion/deletion RNA editing in trypanosome mitochondria: A review. Gene. 240, 247–260.

    Article  Google Scholar 

  7. Dombrowski S., Brennicke A., Binder S. 1997. 3′-Inverted repeats in plant mitochondrial mRNAs are processing signals rather than transcription terminators. EMBO J. 16, 5069–5076.

    Google Scholar 

  8. Blum B., Simpson L. 1990. Guide RNAs in kinetoplastid mitochondria have a noncoded 3′ oligo(U) tail involved in recognition of the preedited region. Cell. 62, 391–397.

    Google Scholar 

  9. Tarassoff I.A., Levchenko I.V., Zaitseva G.N. 1987. Transcripts of the maxicircle kinetoplast DNA of Crithidia oncopelti. Mol. Biochem. Parasitol. 26, 235–245.

    Google Scholar 

  10. Koslowsky D.J., Yahampath G. 1997. Mitochondrial mRNA 3′ cleavage/polyadenylation and RNA editing in Trypanosoma brucei are independent events. Mol. Biochem. Parasitol. 90, 81–94.

    Google Scholar 

  11. Salavati R., Ranigrahi A., Stuart K. 2001. Mitochondrial ribonuclease P activity of Trypanosoma brucei. Mol. Biochem. Parasitol. 115, 109–117.

    Google Scholar 

  12. LeBlank A., Yermovsky-Kammerer A., Hajduk S. 1999. A nuclear encoded and mitochondrial imported dicistronic tRNA precursor in Trypanosoma brucei. J. Biol. Chem. 274, 21071–21077.

    Google Scholar 

  13. Militello K.T., Read L. 1999. Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: Complete editing is not required for long poly(A) tract addition. Nucleic Acids Res. 27, 1377–1385.

    Google Scholar 

  14. Ochs D.E., Otsu K., Teixeira S.M.R., Moser D.R., Kirchhoff L.V. 1996. Maxicircle genomic organization and editing of an ATPase subunit 6 RNA in Trypanosoma cruzi. Mol. Biochem. Parasitol. 76, 267–278.

    Google Scholar 

  15. Maslov D.A., Avila H.A., Lake J.A., Simpson L. 1994. Evolution of the RNA editing in kinetoplastid protozoa. Nature. 368, 345–348.

    Google Scholar 

  16. Yasuhira S., Simpson L. 1995. Minicircle-encoded guide RNAs from Crithidia fasciculata. RNA. 1, 634–643.

    Google Scholar 

  17. Maslov D.A., Entelis N.S., Kolesnikov A.A., Zaitseva G.N. 1982. Kinetoplast DNA of Crithidia oncopelti: Restriction mapping of maxicircular DNA. Bioorg. Khim. 8, 676–685.

    Google Scholar 

  18. Sambrook J., Fritch T., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Lab. Press.

    Google Scholar 

  19. Li M., Mihara K. 2001. Insertion of mitochondrial DNA-encoded F1 F0-ATPase subunit 8 across the mitochondrial inner membrane in vitro. J. Biol. Chem. 276, 24704–24712.

    Google Scholar 

  20. Okimoto R., Macfarlance J.L., Wolsteholme D.R. 1990. Evidence for the frequent use of TTG as the translation initiation codon of mitochondrial protein genes in the nematodes, Ascaris suum and Caenorhabditis elegans. Nucleic Acids Res. 18, 6113–6118.

    Google Scholar 

  21. Himeno H., Masaki H., Kawai T., Ohta T., Kumagai I., Miura K., Watande K. 1987. Unusual genetic codes and a novel gene structure for tRNA(AGYSer) in starfish mitochondrial DNA. Gene. 56, 219–230.

    Google Scholar 

  22. Jukes T.H., Osawa S. 1990. The genetic code in mitochondria and chloroplasts. Experientia. 46, 1117–1126.

    Google Scholar 

  23. Marienfeld J., Unseld M., Brandt P., Brennicke A. 1996. Genomic recombination of the mitochondrial atp6 gene in Arabidopsis thaliana at the protein processing site creates two different presequences. DNA Res. 31, 287–290.

    Google Scholar 

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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 1, 2005, pp. 61–66.

Original Russian Text Copyright © 2005 by Bessolitsina, Fedyakov, Merzlyak, Kolesnikov.

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Bessolitsina, E.A., Fedyakov, A.V., Merzlyak, E.M. et al. The ATPase subunit 6 gene of Leptomonas seymouri (Trypanosomatidae) is transcribed and edited as a polycistronic mRNA. Mol Biol 39, 53–57 (2005). https://doi.org/10.1007/s11008-005-0007-y

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  • DOI: https://doi.org/10.1007/s11008-005-0007-y

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