Molecular Biology

, Volume 39, Issue 1, pp 53–57 | Cite as

The ATPase subunit 6 gene of Leptomonas seymouri (Trypanosomatidae) is transcribed and edited as a polycistronic mRNA

  • E. A. Bessolitsina
  • A. V. Fedyakov
  • E. M. Merzlyak
  • A. A. Kolesnikov
Genomics. Transcriptomics. Proteomics


The maturation of mRNA was studied for the Leptomonas seymouri mitochondrial A6 gene coding for ATPase subunit 6. RT-PCR showed that the cytochrome b and A6 genes are transcribed to produce a polycistronic RNA. The RT-PCR products, containing the cDNAs of these genes, were cloned and sequenced. Comparison of the sequence for 12 clones revealed four types of transcripts representing partly or completely edited products of A6. Two different variants of completely edited A6 mRNAs were found.

Key words

RNA editing Trypanosomatidae kinetoplast polycistronic RNA pre-mRNA maturation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Stiles J., Micock P., Shah P., Meade J. 1999. Genomic organization, transcription, splicing and gene regulation in Leishmania. Ann. Trop. Med. Parasitol. 93, 781–807.Google Scholar
  2. 2.
    Clayton D.A. 2000. Vertebrate mitochondrial DNA: A circle of surprises. Exp. Cell Res. 255, 4–9.Google Scholar
  3. 3.
    Reichert A., Rothbauer U., Merl M. 1998. Processing and editing of overlapping tRNA in human mitochondria. J. Biol. Chem. 273, 31977–31984.Google Scholar
  4. 4.
    Brennicke A., Marchfelder A., Binder S. 1998. RNA editing. FEMS Microbiol. Rev. 23, 297–316.Google Scholar
  5. 5.
    Alfonzo J.D., Thiemann O., Simpson L. 1997. The mechanism of U insertion/deletion RNA editing in kine-toplastid mitochondria. Nucleic Acids Res. 25, 3751–3759.Google Scholar
  6. 6.
    Estevez A.M., Simpson L. 1999. Uridine insertion/deletion RNA editing in trypanosome mitochondria: A review. Gene. 240, 247–260.CrossRefGoogle Scholar
  7. 7.
    Dombrowski S., Brennicke A., Binder S. 1997. 3′-Inverted repeats in plant mitochondrial mRNAs are processing signals rather than transcription terminators. EMBO J. 16, 5069–5076.Google Scholar
  8. 8.
    Blum B., Simpson L. 1990. Guide RNAs in kinetoplastid mitochondria have a noncoded 3′ oligo(U) tail involved in recognition of the preedited region. Cell. 62, 391–397.Google Scholar
  9. 9.
    Tarassoff I.A., Levchenko I.V., Zaitseva G.N. 1987. Transcripts of the maxicircle kinetoplast DNA of Crithidia oncopelti. Mol. Biochem. Parasitol. 26, 235–245.Google Scholar
  10. 10.
    Koslowsky D.J., Yahampath G. 1997. Mitochondrial mRNA 3′ cleavage/polyadenylation and RNA editing in Trypanosoma brucei are independent events. Mol. Biochem. Parasitol. 90, 81–94.Google Scholar
  11. 11.
    Salavati R., Ranigrahi A., Stuart K. 2001. Mitochondrial ribonuclease P activity of Trypanosoma brucei. Mol. Biochem. Parasitol. 115, 109–117.Google Scholar
  12. 12.
    LeBlank A., Yermovsky-Kammerer A., Hajduk S. 1999. A nuclear encoded and mitochondrial imported dicistronic tRNA precursor in Trypanosoma brucei. J. Biol. Chem. 274, 21071–21077.Google Scholar
  13. 13.
    Militello K.T., Read L. 1999. Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: Complete editing is not required for long poly(A) tract addition. Nucleic Acids Res. 27, 1377–1385.Google Scholar
  14. 14.
    Ochs D.E., Otsu K., Teixeira S.M.R., Moser D.R., Kirchhoff L.V. 1996. Maxicircle genomic organization and editing of an ATPase subunit 6 RNA in Trypanosoma cruzi. Mol. Biochem. Parasitol. 76, 267–278.Google Scholar
  15. 15.
    Maslov D.A., Avila H.A., Lake J.A., Simpson L. 1994. Evolution of the RNA editing in kinetoplastid protozoa. Nature. 368, 345–348.Google Scholar
  16. 16.
    Yasuhira S., Simpson L. 1995. Minicircle-encoded guide RNAs from Crithidia fasciculata. RNA. 1, 634–643.Google Scholar
  17. 17.
    Maslov D.A., Entelis N.S., Kolesnikov A.A., Zaitseva G.N. 1982. Kinetoplast DNA of Crithidia oncopelti: Restriction mapping of maxicircular DNA. Bioorg. Khim. 8, 676–685.Google Scholar
  18. 18.
    Sambrook J., Fritch T., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Lab. Press.Google Scholar
  19. 19.
    Li M., Mihara K. 2001. Insertion of mitochondrial DNA-encoded F1 F0-ATPase subunit 8 across the mitochondrial inner membrane in vitro. J. Biol. Chem. 276, 24704–24712.Google Scholar
  20. 20.
    Okimoto R., Macfarlance J.L., Wolsteholme D.R. 1990. Evidence for the frequent use of TTG as the translation initiation codon of mitochondrial protein genes in the nematodes, Ascaris suum and Caenorhabditis elegans. Nucleic Acids Res. 18, 6113–6118.Google Scholar
  21. 21.
    Himeno H., Masaki H., Kawai T., Ohta T., Kumagai I., Miura K., Watande K. 1987. Unusual genetic codes and a novel gene structure for tRNA(AGYSer) in starfish mitochondrial DNA. Gene. 56, 219–230.Google Scholar
  22. 22.
    Jukes T.H., Osawa S. 1990. The genetic code in mitochondria and chloroplasts. Experientia. 46, 1117–1126.Google Scholar
  23. 23.
    Marienfeld J., Unseld M., Brandt P., Brennicke A. 1996. Genomic recombination of the mitochondrial atp6 gene in Arabidopsis thaliana at the protein processing site creates two different presequences. DNA Res. 31, 287–290.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • E. A. Bessolitsina
    • 1
  • A. V. Fedyakov
    • 1
  • E. M. Merzlyak
    • 1
  • A. A. Kolesnikov
    • 1
  1. 1.Biological FacultyMoscow State UniversityMoscowRussia

Personalised recommendations