Insilico Studies on Antimicrobial Peptide (AMP) in Leeches

Abstract

The earthworm immune system is robust and comprises of the coelom cytolytic factor (CCF), lysenin and antimicrobial peptides (AMPs, Ghosh in Environ Sci Pollut Res 25: 6196, 2018) have been reported from organisms belonging to Phylum Annelida including earthworms (Ghosh in Int J Pept Res Ther, http://doi.org/10.1007/s10989-019-09970-9, 2019) which reveal structural diversity. Leeches have been known of their medical importance and known to produce AMPs (Hung et al. in J Proteomics 103:216–226, 2014; Salzet Curr Med Chem 12(6):3055–3061, 2005), but information on their detailed structures were not observed in published literature. In this study we have conducted insilico studies to understand the (i) Physicochemical properties (ii) phylogenetic relation from peptide sequence (iii) molecular modelling studies by insilico approaches which reveals overall diversity in their structure despite their similar functional role. This report highlights the properties of all AMPs in leeches with known sequences and the importance of application of insilico tools, performing a mutation analysis affecting protein stability, to highlight the significance of K(8) amino acid of the AMP 536_2 from Hirudo medicinalis in maintaining protein stability and functional importance.

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Acknowledgements

The author acknowledges NISER Bhubaneswar for the study.

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Correspondence to Shyamasree Ghosh.

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Not applicable as it is an insilico study

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Not applicable since it is an insilico study.

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Ghosh, S. Insilico Studies on Antimicrobial Peptide (AMP) in Leeches. Int J Pept Res Ther 26, 2253–2267 (2020). https://doi.org/10.1007/s10989-020-10017-7

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Keywords

  • Leech
  • Antimicrobial peptide (AMP)
  • Molecular modelling