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The Variations, Including Structures and Attenuation to Hemolysis, of Peptide Purified from Dendrobium aphyllum During In Vitro Gastro-Intestinal Digestion and Caco-2 Uptake and Transportation

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Abstract

In a previous study a crude peptide extract was obtained from Dendrobium aphyllum, and this study focused on the functional characterization of the peptide fraction (named DPP) after being purified by DEAE Sepharose. The DPP with high purity contained 16 types of free amino acids and 6 polypeptides. DPP could act as the AAPH-free radical scavenger to protect the erythrocyte under oxidative stress, and the attenuation significantly increased after intestinal digestion. Moreover, the results of the Caco-2 cell models indicated the high absorptive and transfer rates of DPP, in which the passive diffusion was the major transfer pathway. Through the LC-TOF/MS-MS analysis, the peptide sequences Asp-Asp-Asp-Tyr and Asp-Tyr-Asp-Asp were very sensitive to the gastric environment and were completely degraded by Caco-2 cells. While Ser-Ser-Arg, Ala-Val-Thr-Phe, and Met-Leu-Cys-Ala resisted the gastric condition; and sequence Met-Leu-Cys-Ala maintained its structure after the subsequent intestinal digestion and the Caco-2 cells’ uptake.

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Funding

This study was funded by the Guangzhou Science and Technology Program of China (Project No. 201704030084), and the Guangdong Science and Technology Program of China (Project No. 2016A040402045).

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Authors and Affiliations

  1. College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China

    Huifan Liu & Qin Wang

  2. College of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, Guangdong, China

    Hui Wu

Authors
  1. Huifan Liu
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  2. Hui Wu
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  3. Qin Wang
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Correspondence to Qin Wang.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Scientific Ethic Committee at South China University of Technology and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Liu, H., Wu, H. & Wang, Q. The Variations, Including Structures and Attenuation to Hemolysis, of Peptide Purified from Dendrobium aphyllum During In Vitro Gastro-Intestinal Digestion and Caco-2 Uptake and Transportation. Int J Pept Res Ther 25, 1319–1331 (2019). https://doi.org/10.1007/s10989-018-9778-6

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  • DOI: https://doi.org/10.1007/s10989-018-9778-6

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