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Sea Anemones as Potential Source for Bioactive Metabolites

  • S. Thangaraj
  • S. Bragadeeswaran
  • V. Gokula
Article
  • 65 Downloads

Abstract

Marine organisms are novel sources for biologically active compounds which are potentially valuable materials in biomedical research. In the present investigation, the potential bioactive compounds were isolated from the sea anemone Heteractis aurora collected from Mandapam, Southeast coast India. The maximum inhibition zone was found against bacterial pathogens (Klebsiella oxytoca 7.2 ± 1.5 and Escherichia coli 8.1 ± 0.2) followed by fungal pathogens (Botrytis cinerea 5.3 ± 0.5 and Trichoderma koning 4.2 ± 1.2). The antioxidant activity was found to be 42.2 ± 1.14%, whereas hemolytic activity was recorded as 64 Hemolytic unit against chicken blood erythrocytes. The chemical characterizations of sea anemone extract were carried out by FT-IR, GC–MS and NMR (13C, 1H) spectroscopy. The FT-IR results showed the presence of phenyl ring: C–CH3 and C=C stretching. GCMS analysis revealed the presence of acetic acid-17-acetoxy-4,4,10,13-tetramethyl-7-oxo-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta (a) phenanthren-3-yl (ester) and 4[-4-diethylamino-1-methylbutylamino]-1,2 dimethoxy-6-bromonaphthalene. These identified compounds were subjected for molecular docking analysis against the target protein enoyl-acyl carrier protein reductase which revealed that acetic acid-17-acetoxy-4,4,10,13-tetramethyl-7-oxo-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta (a) phenanthren-3-yl showed better docking interaction than the commercial standard drug Tryptanthrine.

Keywords

Sea anemone Heteractis aurora Hemolytic unit Klebsiella oxytoca Trichoderma koning and Tryptanthrine 

Notes

Acknowledgements

Authors are thankful to Dean & Director, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences and authority of Annamalai University for providing necessary facilities. First author sincerely acknowledged the DBT and UGC-BSR for their financial support.

Funding

Funding was provided by DBT and UGC-BSR projects.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Ethical Approval

The Institutional Ethical Committee of Rajah Muthiah Medical College, Annamalai University, Annamalai Nagar, India (registration number 160/1999/CPCSEA/11.01.2008) approved and provided the ethical clearance for the present study.

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Authors and Affiliations

  1. 1.PG & Research Department of ZoologyNational CollegeTrichyIndia
  2. 2.CAS in Marine Biology, Faculty of Marine SciencesAnnamalai UniversityParangipettaiIndia

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