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Wound Healing Properties of Commercial Milk Hydrolysates in Intestinal Cells

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Abstract

Eight commercial milk protein preparations of whey protein or casein enzymatic hydrolysates were investigated for migration and proliferation activities in a cell model for wound healing using rat intestinal epithelial cells of normal origin. Two casein-based preparations were very potent stimulators of migration, showing an increase of approximately 50% in concentrations below 1 µg/mL of solubilized milk powder. One of these preparations also stimulated cell proliferation in low concentrations, while the highest level of proliferation was obtained by a third casein-based hydrolysate showing a nearly 50% increase at 1 mg/mL. These results suggest that hydrolysis of casein may be important to obtain functional bioactives in intestinal cell recovery from damage or injury. As migration and proliferation are distinct mechanisms of importance for the integrity of the epithelial surface, both at normal conditions and especially after intestinal injury, these milk protein preparations could be used in functional foods for gut protection and recovery after injury.

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Acknowledgements

The authors thank Anette K. Nielsen, Kasper B. Poulsen, Hanne S. Møller and Stina G. Handberg for their skilled technical assistance. This research was supported by the Danish Council for Strategic Research (Project No. 3304-FSE-06-0508-02) and the Danish Agency for Science, Technology and Innovation.

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Authors and Affiliations

  1. Department of Animal Science, Aarhus University, Blichers Allé 20, P. O. Box 50, 8830, Tjele, Denmark

    Stig Purup

  2. Department of Food Science, Aarhus University, Blichers Allé 20, P. O. Box 50, 8830, Tjele, Denmark

    Søren D. Nielsen, Thao T. Le & Lotte B. Larsen

  3. Arla Foods Ingredients, Sønderhøj 10-12, 8260, Viby J, Denmark

    Hans Bertelsen

  4. Arla Foods Amba, Rørdrumvej 2-10, 8220, Brabrand, Denmark

    John Sørensen

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  1. Stig Purup
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  2. Søren D. Nielsen
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  4. Hans Bertelsen
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  6. Lotte B. Larsen
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Correspondence to Stig Purup.

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Purup, S., Nielsen, S.D., Le, T.T. et al. Wound Healing Properties of Commercial Milk Hydrolysates in Intestinal Cells. Int J Pept Res Ther 25, 483–491 (2019). https://doi.org/10.1007/s10989-018-9692-y

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  • DOI: https://doi.org/10.1007/s10989-018-9692-y

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