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Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines

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Abstract

The LPSBD0 and LPSBD2 peptides, two β-hairpin cationic amphiphilic peptides, exhibit membrane damage and anti-proliferative activities on the A549 (lung cancer) and Hep3B (hepatoma) cell lines were characterized in this study. Light microscopy observations indicate that both peptides induce the production of debris in the cell cultures. The amount of this debris increased in a LPSBD treatment dosage-dependent manner. This debris was also observed by scanning electron microscopy and flow cytometry. As determined by confocal laser microscopy and flow cytometry, cell membrane damage led to Annexin V permeability on the two cancer cell lines used in this study. Both peptide treatments also induced apoptosis in lung and liver cancer cell lines. However, little hemolysis was observed in the hemolytic assay using rat erythrocytes after very-high-dose treatments for both peptides. These results suggest that the two peptides may have the potential to be developed as anti-cancer peptides for human hepatoma and lung cancers.

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Acknowledgments

The research was supported by Ministry of Science and Technology, Taiwan, ROC (Project Grant No. NSC100-2324-B-031-001-CC1).

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

Ethical Standards and Informed Consent

This article does not contain any studies with human participants performed by any of the authors. All procedures performed involving in rat BCs harvest were in accordance with the ethical standards of the institution.

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Authors and Affiliations

  1. Department of Microbiology, Soochow University, Shih-Lin, Taipei, 111, Taiwan, ROC

    Hung-Hung Sung, Po-Ying Fu & Chu-Wen Yang

  2. Biodiversity Research Center and Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan, ROC

    Wann-Neng Jane

  3. Department of Pharmacology, Institute for Drug Evaluation Platform, Development Center for Biotechnology, New Taipei City, Taiwan, ROC

    Wen-Yi Kao & Shih-Ching Ho

Authors
  1. Hung-Hung Sung
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  2. Wann-Neng Jane
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Correspondence to Chu-Wen Yang.

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Sung, HH., Jane, WN., Kao, WY. et al. Lipopolysaccharide-Binding Motif Derived Peptides Induce Cell Membrane Damages in Human Lung Cancer and Hepatoma Cell Lines. Int J Pept Res Ther 21, 313–324 (2015). https://doi.org/10.1007/s10989-015-9459-7

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  • DOI: https://doi.org/10.1007/s10989-015-9459-7

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