A bFGF Antagonist Peptide with Anti-angiogenesis Properties in Non-small Cell Lung Cancer Cells
Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality worldwide. Basic fibroblast growth factor (bFGF) is up-regulated in NSCLC patients and plays an important role in tumor growth and angiogenesis. Therefore, it is regarded as a potential therapeutic target of NSCLC. We have previously obtained a high-affinity bFGF antagonist peptide (named P7) with the potential of suppressing angiogenesis stimulated by bFGF from the phage display random heptapeptide library. Herein, we further revealed the underlying anti-angiogenic mechanisms of P7 peptides in human NSCLC cells. P7 not only inhibited the expressions of angiogenesis related factors including VEGF, MMP-2 and MMP-9 at both transcriptional and translational levels, but also induced significant changes in the expressions of proteins related to tumor growth and progress. Our results suggested that P7 peptides with potent anti-angiogenic activity may have therapeutic potential in NSCLC.
KeywordsbFGF antagonist peptide Angiogenesis Proteome Non-small cell lung cancer
This work was supported by Grants from the National Natural Science Foundation of China (30973671, 81071800), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, the Guangdong Provincial Science and Technology Program (2010B060900040), the Natural Science Foundation of Guangdong Province of China (9151064001000031), the Natural Science Foundation of Zhejiang Province of China (Y2090292, Y4090379), the Science and Technology Planning Project of Wenzhou (Y20090244), the Fundamental Research Funds for the Central Universities (X. P. Wu), Guangdong Provincial ‘‘Thousand-Hundred-Ten Talent Project’’ (X. P. Wu), and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Jinan University.
Conflict of interest
The authors declare no conflicts of interest.
- Lund EL, Thorsen C, Pedersen MW, Junker N, Kristjansen PE (2000) Relationship between vessel density and expression of vascular endothelial growth factor and basic fibroblast growth factor in small cell lung cancer in vivo and in vitro. Clin Cancer Res 6:4286–4291Google Scholar
- Tu SH, Chang CC, Chen CS, Tam KW, Wang YJ, Lee CH, Lin HW, Cheng TC, Huang CS, Chu JS, Shih NY, Chen LC, Leu SJ, Ho YS, Wu CH (2010) Increased expression of enolase alpha in human breast cancer confers tamoxifen resistance in human breast cancer cells. Breast Cancer Res Treat 121:539–553PubMedCrossRefGoogle Scholar