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The Monocyte Locomotion Inhibitory Factor (MLIF) Produced by Entamoeba histolytica Alters the Expression of Genes Related to the Wound-Healing Process

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Abstract

The monocyte locomotion inhibitory factor (MLIF) is a pentapeptide produced by Entamoeba histolytica. This factor displays several in vivo and in vitro anti-inflammatory properties, among others, inhibition of monocyte locomotion and the respiratory burst in monocyte and neutrophils. A synthetic peptide had the same selective anti-inflammatory features as the native material. We now evaluated MLIF on the constitutive and induced gene expression in the MRC-5 human fibroblasts cell line. The MLIF affected constitutive expression of 21 genes and induced expression of 75 genes. Some of these genes involved in the inflammatory response and other participated in the remodeling and the wound-healing processes.

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Acknowledgments

The authors wished to thank Diego Adrián Oliva Rico from the Licenciatura en Investigación Biomédica, UNAM, for the comments and critical review of the manuscript.

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Authors and Affiliations

  1. Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, CMN-Siglo XXI IMSS. Av., Cuauhtémoc # 330, CP. 06720, Mexico, DF, Mexico

    R. Silva-García, M. E. Morales-Martínez, F. Blanco-Favela, J. Ríos-Olvera, M. R. Garrido-Ortega & E. Tesoro-Cruz

  2. Instituto de Biotecnología Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico

    A. Torres-Salazar

  3. División de Investigación, Facultad de Medicina, UNAM. Av., Universidad 3000 Circuito Escolar s/n, Col. Copilco, Delegación Coyoacán, CP. 04510, Mexico, DF, Mexico

    G. Rico-Rosillo

  4. Departamento de Investigación, Experimental y Bioterio del I.N.C.M.N “SZ”, Vasco de Quiroga 15. Col Talalpan, Mexico, DF, Mexico

    E. Tesoro-Cruz

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  1. R. Silva-García
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  2. M. E. Morales-Martínez
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  3. F. Blanco-Favela
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Correspondence to R. Silva-García.

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Silva-García, R., Morales-Martínez, M.E., Blanco-Favela, F. et al. The Monocyte Locomotion Inhibitory Factor (MLIF) Produced by Entamoeba histolytica Alters the Expression of Genes Related to the Wound-Healing Process. Int J Pept Res Ther 18, 391–401 (2012). https://doi.org/10.1007/s10989-012-9315-y

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