Glutathione (GSH) Determination by a Very Simple Electrochemical Method
Glutathione (GSH; gamma-glutamylcysteinylglycine) is ubiquitous in mammalian and other living cells. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. It has several important functions, including protection against oxidative stress. It is synthesized from its constituent amino acids by the consecutive actions of gamma-glutamylcysteine synthetase and GSH synthetase. Cellular levels of GSH may be increased by supplying substrates and GSH delivery compounds. Increasing cellular GSH may be therapeutically useful. In this study, we investigated the applicability of the glassy carbon electrode coated with thin Hg film layer to the determination of reduced glutathione (GSH). For this purpose, firstly, Hg coating process parameters were studied such as concentration of mercury coating solution, coating current, coating temperature. Then, working conditions were investigated. At the end of these studies, we concluded that although some of limitations, the sensor would be applicable to the determination of reduced glutathione.
KeywordsGlutathione Thin Hg films GSH Biosensor Mercury film electrode
- Pastore A, Piemonte F, Locatelli M, Lo Russo A, Gaeta LM, Tozzi G, Federici G (2003) Determination of blood total, reduced, and oxidized glutathione in pediatric subjects. Clin Chem 47(8):1467–1469Google Scholar
- Patterson AD, Li H, Eichler GS, Krausz KW, Weinstein JN, Fomace AJ, Gonzalez FJ, Idle JR (2008) UPLC-ESI-TOFMS-based metabolomics and gene expression dynamics inspector self-organizing metabolomic maps as tools for understanding the cellular response to ionizing radiation. Anal Chem 80:665–674PubMedCrossRefGoogle Scholar
- Potapenko DI, Bagryanskaya EG, Grigoriev IA, Maksimov AM, Reznikov VA, Platonov VE, Clanton TL, Khramtsov VV (2005) Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2, 3, 5, 6-tetrafluoro-4-mercaptobenzoic acid. Magn Reson Chem 43:902–909PubMedCrossRefGoogle Scholar