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A Synthetic Peptide Based on a Natural Salivary Protein Reduces Demineralisation in Model Systems for Dental Caries and Erosion

  • Jelena Kosoric
  • Ralph Anthony D. Williams
  • Mark P. Hector
  • Paul Anderson
Special Issue: Peptides in Oral and Dental Research

The salivary protein statherin is an inhibitor of spontaneous and secondary precipitation of hydroxyapatite (HAp). It is also detected in enamel pellicle. The N-terminal region of statherin is involved in its adsorption onto tooth surfaces, and, calcium binding. A peptide (StN21) was designed with a 21 amino acid sequence identical to the N-terminus of statherin. The aim was to measure the effect of StN21 on the rate of mineral loss in a model system for dental caries and erosion using HAp subjected to artificial carious and erosive conditions. StN21 was synthesised using Fmoc chemistry. A surface of each HAp block was exposed to solution containing StN21 at concentrations 9.4–376 μmol L−1 (in phosphate buffer) for 24 h. Controls were HAp exposed to buffer only, and HAp exposed to lysozyme. Demineralising solution (0.1 mol L−1 acetic acid, pH 4.5, 1.0 mmol L−1 calcium and 0.6 mmol L−1 phosphate) was circulated past the HAp blocks at 0.4 mL min-1 to mimic carious and erosive conditions. Scanning microradiography was used to measure the rate of mineral loss for demineralisation periods of 3 weeks. The rate of mineral loss of the samples exposed to StN21 was reduced by ∼40% compared to the controls, but no dependence on the concentration of StN21 was observed at the concentrations used. StN21 has been shown to be a potent and stable peptide that has potential as a preventive/therapeutic agent in the treatment of enamel erosion and dental caries.

Keywords

statherin dentistry saliva demineralisation hydroxyapatite 

Abbreviations

XMT

X-ray microtomography

HBTU

[2-(1H-benzotriazol-1yl)-1,1,3,3-tetramethyl-uronium hexafluorophosphate] HOBt (N-hydroxybenzotriazol)

MBTE

methyl-tertiary butylether

TFA

trifluoracetic acid

Notes

Acknowledgment

JK gratefully acknowledges financial support through a Wellcome VIP award.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jelena Kosoric
    • 1
  • Ralph Anthony D. Williams
    • 1
  • Mark P. Hector
    • 1
  • Paul Anderson
    • 1
  1. 1.Centre for Oral Growth and DevelopmentQueen Mary’s School of Medicine and DentistryLondonUK

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